Goat anti-Guinea Pig IgG1 (Subclass specific) Antibody (Biotin) - Preadsorbed

Catalog No. ABIN457757

Quick Overview for Goat anti-Guinea Pig IgG1 (Subclass specific) Antibody (Biotin) - Preadsorbed (ABIN457757)

Target: IgG1

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Isotype: IgG

Product Details

Binding Specificity: Subclass specific

Reactivity: Guinea Pig

Host: Goat

Purpose: Goat anti Guinea Pig IgG (subclass specific), conjugated with Biotin

Specificity: Biotin-conjugated IgG fraction of polyclonal Goat antiSerum to Guinea Pig IgG1, subclass specific

Cross-Reactivity (Details): This immunoconjugate is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this antiSerum has not been tested in detail.

Characteristics: The reactivity of the antiserum is directed to the subclass IgG1. It does not react with IgG2, IgG/Fab fragments, IgM and IgA or any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunocytochemical and immunohistochemical staining for the detection of IgG1 at the cellular and subcellular level of appropriately treated cell and tissue substrates, to demonstrate circulating IgG1 antibodies in serodiagnostic microbiology and autoimmune diseases, to identify a specific antigen using a reference antibody of guinea pig origin known to be of the IgG1 isotype in the middle layer of the indirect test procedure, in non-isotopic assay methodology (e.g. ELISA) to measure IgG1 in guinea pig serum or other body fluids. As a second step an avidin or streptavidin conjugate of the user's choice has to be used This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified

Immunogen: Purified IgG1 isolated from guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Biotin-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

References

Lee, Park, Ko, Lee, Lee, Kim, Nam: "Evaluation of glycoprotein E subunit and live attenuated varicella-zoster virus vaccines formulated with a single-strand RNA-based adjuvant." in: Immunity, inflammation and disease, Vol. 8, Issue 2, pp. 216-227, (2021) (PubMed).

Xia, Veselenak, Gorder, Bourne, Milligan: "Virus-specific immune memory at peripheral sites of herpes simplex virus type 2 (HSV-2) infection in guinea pigs." in: PLoS ONE, Vol. 9, Issue 12, pp. e114652, (2016) (PubMed).

Target Details

Target: IgG1

Target Type: Antibody