Sheep anti-Guinea Pig IgG1 (Subclass specific) Antibody

Catalog No. ABIN457777

Quick Overview for Sheep anti-Guinea Pig IgG1 (Subclass specific) Antibody (ABIN457777)

Target: IgG1

Clonality: Polyclonal

Application: Immunoelectrophoresis (IEP), Ouchterlony Double Diffusion (ODD)

Isotype: IgG

Product Details

Binding Specificity: Subclass specific

Reactivity: Guinea Pig

Host: Sheep

Purpose: Sheep anti Guinea Pig IgG1 (subclass specific)

Specificity: Precipitating polyclonal Sheep antiSerum to Guinea Pig IgG1, subclass specific.

Cross-Reactivity (Details): This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. Cross-reactivity of this product has not been tested in detail.

Characteristics: The reactivity of the antiserum is directed to the Fc subunit of subclass IgG1. It does not react with other IgG2, IgG/Fab fragments, IgM and IgA or any non-Ig protein in guinea pig serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG1 in guinea pig serum and other body fluids or to determine its concentration.

Purification: Delipidated, heat inactivated lyophilized stable whole serum

Immunogen: Pools of purified normal IgG1 isolated from pooled guinea pig serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Precipitation assays. In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized whole antiserum. No preservative added, as it may interfere with the antibody activity. Total protein and IgG concentration in the antiserum are comparable to those of pooled normal sheep serum. No foreign protei

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details

Target: IgG1

Target Type: Antibody