Mouse anti-Human IgM Antibody

Catalog No. ABIN457833

Quick Overview for Mouse anti-Human IgM Antibody (ABIN457833)

Target: IgM

Clonality: Monoclonal

Application: ELISA, Immunofluorescence (IF)

Clone: NI 179

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Specificity: The reactivity of the antiserum is restricted to an isotype specific determinant on the IgM molecule as tested in ELISA, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cyto-plasmic Ig. Purified monoclonal mouse antibody to human IgM, isotype specific

Characteristics: Purified monoclonal mouse antibody to human IgM, isotype specific

Purification: Purified

Immunogen: Highly purified monoclonal IgM isolated from pooled human serum

Application Details

Application Notes: To identify the presence of IgM in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgM immunoblotting.
The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200. These data should be interpreted as general recommendations only.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized ascites by adding 0. 5 mL sterile distilled water.

Concentration: 0.8 mg/mL

Buffer: Purified mouse IgG1 kappa lyophilized from a solution in phosphate buffered saline (pH 7.2).

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below - 24 °C.

Target Details

Target: IgM

Target Type: Antibody