Mouse anti-Human IgG4 (pFc' Region) Antibody (FITC)

Catalog No. ABIN457903

Product Details

Target: IgG4

Binding Specificity: pFc' Region

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: FITC

Application: Western Blotting (WB), ELISA

Specificity: The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc part of the IgG4 molecule as tested in direct binding enzyme immunoassay, immunoblotting, immuno-precipitation and direct immunoperoxidase staining of cytoplasmic Ig.

Characteristics: Fluorescein isothiocyanate-conjugated purified monoclonal mouse antibody to human IgG4, subclass specific
Fluorochrome/IgG protein molar ratio (F/P) approximately 1.5.

Purification: Purified

Immunogen: Highly purified monoclonal IgG4 isolated from human serum.

Clone: NI 315 (HP 6206)

Isotype: IgG1 kappa

Application Details

Application Notes: To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase staining of cytoplasmic IgG4, and immunoblotting using a peroxidase labelled monoclonal antibody against FITC.
The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:50, in ELISA from 1:200 upwards, in Western blotting from 1:400 upwards. Working dilutions may vary widely, strongly depending on the test conditions. These data should be interpreted as general recommendations only.

Comment:

Excitation/Emission wavelength: 494 nm/514 nm

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0. 5 mL sterile distilled water.

Concentration: 0.4 mg/mL

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with FITC, lyophilized from a solution in phosphate buffered saline (pH 7.2).

Preservative: Without preservative

Handling Advice: Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7. 2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4 °C, not refrozen, and preferably used the same day.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: IgG4

Alternative Name: Immunoglobulin G4 (IgG4) (IgG4 Products)

Target Type: Antibody