Mouse anti-Human IgA2 (Allotype IgA2(m)2) Antibody

Catalog No. ABIN457924

Quick Overview for Mouse anti-Human IgA2 (Allotype IgA2(m)2) Antibody (ABIN457924)

Target: IgA2

Clonality: Monoclonal

Application: ELISA, Immunofluorescence (IF)

Clone: NI 194-3 (A89-040)

Isotype: IgG1

Product Details

Binding Specificity: Allotype IgA2(m)2

Reactivity: Human

Host: Mouse

Specificity: Monoclonal Mouse antiSerum to Human IgA2(m)2, allotype specific

Cross-Reactivity (Details): The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.

Characteristics: The reactivity of the antiserum is restricted to the IgA2(m)2 Molecule as tested in haemagglutination, haemagglutination inhibition, direct binding enzyme immunoassay, competitive inhibition enzyme immunoassay, immunoblotting, immunoprecipitation, latex agglutination assay and histochemistry (Results of an IUIS/WHO collaborative study, Mestecky J. et al. (1996) J. Immunol. Methods 193, 103-148). To identify the presence of IgA2(m)2 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as radio immuno assay, ELISA, indirect immunoperoxidase and immunofluorescence staining of cytoplasmic IgA2(m)2, haemagglutination and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:200 upwards, in Western blotting from 1:500 upwards. These data should be interpreted as general recommendations only.

Purification: Delipidated, heat inactivated, stable ascites

Sterility: Heat-inactivated

Immunogen: Highly purified monoclonal IgA2(m)2 isolated from human serum.

Application Details

Application Notes: Indirect immunofluorescence,ELISA.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/mL. No foreign proteins added. Reconstitute the lyophilized ascites by adding 0.5 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole ascites IgG concentration is 1 mg/mL. No foreign proteins added.

Storage: RT,4 °C,-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details

Target: IgA2

Alternative Name: IgA22