Mouse anti-Human IgG3 (Allotype G3m(U)) Antibody

Catalog No. ABIN457932

Quick Overview for Mouse anti-Human IgG3 (Allotype G3m(U)) Antibody (ABIN457932)

Target: IgG3

Clonality: Monoclonal

Application: ELISA, Immunofluorescence (IF)

Clone: NI 86 (HP 6080)

Isotype: IgG1

Product Details

Binding Specificity: Allotype G3m(U)

Reactivity: Human

Host: Mouse

Specificity: Monoclonal Mouse antibody to Human IgG3m(u), allotype specific.

Cross-Reactivity (Details): The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.

Characteristics: The reactivity of the antiserum is restricted to an allotype specific determinant of the IgG3m(u) molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3m(u) in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG3m(u), and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.

Purification: Delipidated, heat inactivated, stable ascites

Sterility: Heat-inactivated

Immunogen: Highly purified monoclonal IgG3m(u) isolated from human serum

Application Details

Application Notes: Indirect immunofluorescence,ELISA.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration after solution is 1.0 mg/mL. No foreign proteins added. Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.

Buffer: Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. IgG concentration after solution is 1.0 mg/mL. No foreign proteins added.

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details

Target: IgG3

Target Type: Antibody