Mouse anti-Human IgG3 (Allotype G3m(U)) Antibody (Biotin)

Catalog No. ABIN457898

Product Details

Target: IgG3

Binding Specificity: Allotype G3m(U)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Biotin

Application: ELISA, Western Blotting (WB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Dot Blot (DB)

Specificity: Biotin-conjugated purified monoclonal Mouse antibody to Human IgG3m(u), allotype specific.

Cross-Reactivity (Details): The antiSerum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species.

Characteristics: The reactivity of the antiserum is restricted to the allotype specific determinant on the IgG3 Molecule as tested in direct binding enzyme immunoassay, immunoblotting, immunoprecipitation and direct immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG3m(u) in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, immunoperoxidase staining of cytoplasmic IgG3, and immunoblotting. As a second step an avidin or streptavidin conjugate of the customer's choice have to be used. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:100 to 1:500, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards.

Purification: Purified

Immunogen: Highly purified monoclonal IgG3 isolated from human serum.

Clone: NI 86 (HP 6080)

Isotype: IgG1

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 0.5 mL sterile distilled water.

Buffer: Purified monoclonal mouse IgG1 kappa conjugated with biotin, lyophilized from a solution in phosphate buffered saline ( pH 7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details

Target: IgG3

Abstract: IgG3 Products

Target Type: Antibody