Goat anti-Human IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN457953

Quick Overview for Goat anti-Human IgG (Heavy & Light Chain) Antibody (ABIN457953)

Target: IgG

Clonality: Polyclonal

Application: Immunoprecipitation (IP)

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Goat

Specificity: Precipitating polyclonal Goat antiSerum to Human IgG, heavy and light chains

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. This antiSerum has been tested for cross-reactivity in double radial immunodiffusion with the following results: Bovine - Dog + Horse ± pigeon - Sheep - Cat + Guinea Pig + Monkey ++ Rabbit + Swine + Chicken - Hamster ± Mouse ± Rat + Turkey -

Characteristics: The reactivity of the antiserum is directed to the Fc and Fab subunits of IgG. It includes a certain degree of reactivity with other immunoglobulins via the common Fab portion. In immunoelectrophoresis against human serum a single characteristic precipitin line with a spur is usually obtained. It does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. In immunoelectrophoresis and double radial immunodiffusion to identify the presence of IgG, as secondary antibody to precipitate the immunoglobulin in normal human serum, to prepare an immunoadsorbent for the production of Ig-free human serum, plasma or non-Ig preparations. This antiserum is not intended for use in non-precipitating antibody-binding or other highly sensitive assays. For such uses labelled and unlabelled cytochemical and immunoassay reagents meeting the necessary additional specificity and performance requirements are available.

Purification: Delipidated, heat inactivated, stable whole serum

Sterility: Heat-inactivated

Immunogen: Purified normal IgG isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum. No foreign proteins added. It is reconstituted by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat serum.

Preservative: Without preservative

Storage: RT,4 °C,-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: IgG

Target Type: Antibody