Goat anti-Human IgG Antibody (Agarose Beads)
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- Target See all IgG products
- IgG
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Agarose Beads
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Application
- Immunoprecipitation (IP)
- Purification
- This product is an antibody IgG fraction coupled to activated agarose. Sufficient antibody capacity is provided to bind a minimum of 5 mg of pure Human IgG Fc.
- Isotype
- IgG
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- Application Notes
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Application Note: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Immunoprecipitation Dilution: User Optimized
- Restrictions
- For Research Use only
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- Concentration
- 10 mg/mL
- Buffer
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Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
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Do not freeze.
Sensitive to light.
Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume. - Storage
- 4 °C
- Expiry Date
- 12 months
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- Target
- IgG
- Abstract
- IgG Products
- Target Type
- Antibody
- Background
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Synonyms: Goat Anti Human IgG F(c) Antibody Agarose Conjugate, human fc fragment agarose conjugated antibody
Background: Anti-Human IgG F(c) Agarose Conjugated generated in goat detects Human F(c). A proteolytic fragment of immunoglobulin G (IgG) obtained by limited digestion with the enzyme papain under controlled conditions of temperature, time and pH . Receptors bind the Fc portion of human IgG and often this fragment is removed from immunoglobulins to minimize receptor binding and lower background reactivity. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition. F(c) Antibody is ideal for investigators who routinely perform flow cytometry, immunohistochemistry or IHC and other immunoassays.
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