Goat anti-Human Ig (Chain lambda), (Free & Bound) Antibody

Catalog No. ABIN457976

Quick Overview for Goat anti-Human Ig (Chain lambda), (Free & Bound) Antibody (ABIN457976)

Target: Ig

Clonality: Polyclonal

Application: Immunoelectrophoresis (IEP)

Isotype: IgG

Product Details

Binding Specificity: Chain lambda, Free & Bound

Reactivity: Human

Host: Goat

Specificity: The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the lambda type, Bence Jones proteins as well as free light chains of the lambda type. This antiserum does not react with any other protein of human serum or plasmaPrecipitating polyclonal goat antiserum to free and bound human Ig lambda light chain
To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immuno-diffusion.

Characteristics: The reactivity of the antiserum is directed to the surface and hidden determinants of Ig lambda light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the lambda type, Bence Jones proteins as well as free light chains of the lambda type. This antiserum does not react with any other protein of human serum or plasmaPrecipitating polyclonal goat antiserum to free and bound human Ig lambda light chain
To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immuno-diffusion.
Physicochemical characteristic: Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled goat serum. Antibody titre: Precipitin titre not less than 1:32 when tested against normal human serum

Purification: Delipidated, heat inactivated lyophilized whole serum.
Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with human serum proteins.

Immunogen: A pool of purified Bence Jones lambda proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.

Buffer: No foreign proteins added.

Preservative: Without preservative

Handling Advice: Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Repeated thawing and freezing should be avoided.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: Ig