Rabbit anti-Human Ig (Free & Bound) Antibody

Catalog No. ABIN458112

Quick Overview for Rabbit anti-Human Ig (Free & Bound) Antibody (ABIN458112)

Target: Ig

Clonality: Polyclonal

Application: Immunoelectrophoresis (IEP), Ouchterlony Double Diffusion (ODD)

Isotype: IgG

Product Details

Binding Specificity: Free & Bound

Reactivity: Human

Host: Rabbit

Purpose: Rabbit anti Human Ig kappa light chain (free and bound)

Specificity: Precipitating polyclonal Rabbit antiSerum to free and bound Human Ig kappa light chain.

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Characteristics: The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasma. To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immunodiffusion.

Purification: Delipidated, heat inactivated lyophilized whole serum.

Immunogen: A pool of purified Bence Jones kappa proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Precipitation assays. In immunoelectrophoresis use 2 μL serum or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in 3 mm diameter center well and 2 μL serum samples (neat and serially diluted in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.

Buffer: Delipidated, heat inactivated lyophilized whole serum. No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product

Target Details

Target: Ig