Rabbit anti-Human Ig (Chain kappa), (Free & Bound) Antibody

Catalog No. ABIN458112

Product Details

Target: Ig

Binding Specificity: Chain kappa, Free & Bound

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Application: Immunoelectrophoresis (IEP)

Specificity: The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasmaPrecipitating polyclonal rabbit antiserum to free and bound human Ig kappa light chain
To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immuno-diffusion.

Characteristics: To detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immuno-diffusion.
Physicochemical characteristic: Total protein and IgG concentrations in the antiserum are comparable to those of normal pooled rabbit serum. Antibody titre: Precipitin titre not less than 1:32 when tested against normal human serum

Purification: Delipidated, heat inactivated lyophilized whole serum.
Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with human serum proteins.

Immunogen: A pool of purified Bence Jones kappa proteins isolated from human urine. Freund's complete adjuvant is used in the first step of the immunization procedure.

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles.

Buffer: No foreign proteins added.

Preservative: Without preservative

Handling Advice: Working dilutions should be stored at +4 °C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product. Repeated thawing and freezing should be avoided.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4 °C, prolonged storage at or below -24 °C.

Target Details

Target: Ig

Abstract: Ig Products