Goat anti-Human IgA, IgG, IgM (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN457997

Quick Overview for Goat anti-Human IgA, IgG, IgM (Heavy & Light Chain) Antibody (HRP) - Preadsorbed (ABIN457997)

Target: IgA, IgG, IgM

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Western Blotting (WB), Dot Blot (DB), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Human

Host: Goat

Purpose: Goat anti Human IgG IgA IgM (heavy and light chains), conjugated with Horseradish peroxidase

Specificity: Horseradish peroxidase-conjugated IgG fraction of polyclonal Goat antiSerum to Human IgG, IgA and IgM, heavy and light chains

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum with other species has not been tested in detail.

Characteristics: The reactivity of the antiserum is directed to the major isotypes of the human immunoglobulin system (classes and both light chain types) including antibodies to common determinants, to class and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and double radial immunodiffusion using various antiserum concentrations against normal human plasma and serum, the characteristic IgG, IgA and IgM precipitin lines are obtained. In enzyme-immunocytochemical and immunohistochemical staining for the detection of cytoplasmic Ig at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified IgG fraction from antiserum

Immunogen: Purified polyclonal human IgG, and IgA and IgM containing factions isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (paraffin),Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.

Buffer: Peroxidase-coupled purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG + IgA + IgM

Target Type: Antibody