Rabbit anti-Human IgG3 (Subclass specific) Antibody

Catalog No. ABIN458150

Quick Overview for Rabbit anti-Human IgG3 (Subclass specific) Antibody (ABIN458150)

Target: IgG3

Clonality: Polyclonal

Application: Staining Methods (StM), Control Peptide (CP), Ouchterlony Double Diffusion (ODD), Control (Ct)

Isotype: IgG

Product Details

Binding Specificity: Subclass specific

Reactivity: Human

Host: Rabbit

Purpose: Rabbit anti Human IgG3 (subclass specific)

Specificity: Precipitating polyclonal Rabbit antiSerum to Human IgG3, subclass specific.

Cross-Reactivity (Details): The antiSerum does not cross-react with any other component of the Human immunoglobulin system. Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Characteristics: The reactivity of the antiserum is restricted to subclass specific determinants on IgG3. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal human serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG3. No precipitation reaction is obtained with purified other subclasses of IgG, IgA, IgM, and IgG/Fab fragments.

Purification: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Immunogen: Highly purified homogenous IgG3 isolated from human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Precipitation assays. In double radial immunodiffusion (Ouchterlony) serum or plasma samples are prepared in serial two-fold dilutions from 1:8 to 1: 128 and placed in the peripheral wells of the rosette arrangement. One well is filled with pooled human serum or another control. The center wells are filled with the subclass specific antiserum and the sera are allowed to diffuse at ambient temperature for 16-24 hours. Frequently the precipitin lines become visible only after staining.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal rabbit serum. No foreign proteins added.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details

Target: IgG3

Target Type: Antibody