Sheep anti-Human IgA (Fc Region) Antibody (TRITC) - Preadsorbed

Catalog No. ABIN458177

Quick Overview for Sheep anti-Human IgA (Fc Region) Antibody (TRITC) - Preadsorbed (ABIN458177)

Target: IgA

Fragment: F(ab')2 fragment

Clonality: Polyclonal

Conjugate: TRITC

Application: ELISA, Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Indirect Immunofluorescence Assay (IFA)

Isotype: IgG

Product Details

Binding Specificity: Fc Region

Reactivity: Human

Host: Sheep

Purpose: Sheep anti Human IgA (Fc specific), conjugated with TRITC

Specificity: Tetramethylrhodamine isothiocyanate-conjugated F(ab')2 fragments of polyclonal Sheep IgG antibodies to Human IgA, Fc specific.

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this conjugate has not been tested in detail.

Characteristics: The antiserum is reacting with to the Fc subunits of IgA. It does not react with other immunoglobulins or any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. Direct staining of fixed cell and tissue substrates, to demonstrate the intracellular presence of IgA. The absence of the Fc domain in the conjugate ensures minimal interaction with the tissue components and cell surfaces other than the primary antibody activity. This conjugate is primarily intended for use in cell surface membrane staining procedures, to identify and quantitate Ig on B cells, especially if interference by Fc activity is expected.. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Purification: Purified

Immunogen: Purified IgA prepared from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: ELISA,Immunocytochemistry,Immunohistochemistry (frozen),(In)direct immunofluorescence.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: TRITC-coupled sheep F(ab')2 antibodies lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized conjugate is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

Target Details

Target: IgA

Target Type: Antibody