Sheep anti-Mouse IgG (Fab Region) Antibody

Catalog No. ABIN458478

Quick Overview for Sheep anti-Mouse IgG (Fab Region) Antibody (ABIN458478)

Target: IgG

Clonality: Polyclonal

Application: Immunoelectrophoresis (IEP), Ouchterlony Double Diffusion (ODD)

Isotype: IgG

Product Details

Binding Specificity: Fab Region

Reactivity: Mouse

Host: Sheep

Purpose: Sheep anti Mouse IgG Fab

Specificity: Precipitating polyclonal Sheep antiSerum to Mouse Fab of IgG.

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has been not tested in detail.

Characteristics: The antiserum is reacting with the Fab subunit of intact IgG, IgA and IgM and other Ig classes of both light chain types, with their Fab or F(ab')2 subunits and with free light chains of kappa and lambda type as tested in immunoelectrophoresis and double radial immunodiffusion. In precipitating techniques as immunoelectrophoresis and double radial immunodiffusion to identify the presence of Fab, free or bound.

Purification: Delipidated, heat inactivated lyophilized stable whole serum

Immunogen: Purified Fab of normal mouse IgG. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Precipitation assays. In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion (Ouchterlony) use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated lyophilized stable whole serum No preservative added. Total protein and IgG concentration in the antiserum are comparable to those of pooled sheep serum. No foreign proteins added.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: IgG

Target Type: Antibody