Sheep anti-Mouse IgG2a(1a) (Allotype 1a) Antibody

Catalog No. ABIN458480

Quick Overview for Sheep anti-Mouse IgG2a(1a) (Allotype 1a) Antibody (ABIN458480)

Target: IgG2a(1a)

Clonality: Polyclonal

Application: Immunodiffusion (ID), Immunoelectrophoresis (IEP), Immunocytochemistry (ICC)

Isotype: IgG

Product Details

Binding Specificity: Allotype 1a

Reactivity: Mouse

Host: Sheep

Purpose: Sheep anti Mouse IgG2a (1a allotype specific)

Specificity: Precipitating polyclonal Sheep antiSerum to Mouse IgG2a(1a), allotype specific.

Cross-Reactivity (Details): The antiSerum does not cross-react with any other component of the Mouse Ig system. Inter-species cross reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail, expected chance of cross-reactivity will be small.

Characteristics: The reactivity of the antiserum is restricted to the Fc portion of the IgG2a molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against mouse serum of the allelic type Igh-1a, including Balb/C, CBA, AKR and NZB, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgG2a(1a). No precipitation reaction is obtained with purified IgG2a(1b) (C57Bl and SJL/J), other IgG subclasses, IgA, IgG/Fab fragments or other serum proteins. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgG2a(1a) in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgG2a(1a) from serum or plasma. This antiserum is not intended for use in non-precipitating antibodybinding or other highly sensitive assays. For such use labelled an unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity an performance requirements are available.

Purification: Delipidated, heat inactivated, lyophilized, stable whole antiserum

Immunogen: Highly purified normal homogenous IgG2a(1a) isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Precipitation assays.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute the lyophilized antiserum by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized, stable whole antiserum. No preservative added. Total protein and IgG concentrations in the antiserum are comparable to those of pooled normal sheep serum. No foreign proteins added.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the antiserum. Diluted antiserum should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details

Target: IgG2a(1a)