Goat anti-Mouse IgG2 (Subclass specific) Antibody - Preadsorbed

Catalog No. ABIN458515

Quick Overview for Goat anti-Mouse IgG2 (Subclass specific) Antibody - Preadsorbed (ABIN458515)

Target: IgG2

Clonality: Polyclonal

Application: Western Blotting (WB), ELISA, Dot Blot (DB), Indirect Immunofluorescence Assay (IFA)

Isotype: IgG

Product Details

Binding Specificity: Subclass specific

Reactivity: Mouse

Host: Goat

Purpose: Goat anti Mouse IgG2 (subclass specific)

Specificity: IgG fraction of polyclonal Goat antiSerum to Mouse IgG2, subclass specific

Cross-Reactivity (Details): This IgG fraction is not species-specific since inter-species cross-reactivity is a normal feature of antisera to immunoglobulins. However this product has been passed over appropriate immunoadsorbents to remove antibodies cross-reacting with Human immunoglobulins. This renders it specific for use in test systems containing material of Human origin (e.g. Human tissue/Mouse monoclonal antibody to a Human tissue constituent/anti Mouse Ig isotype-specific immunoconjugate. If the IgG fraction is to be used in the presence of material originating from another species, prior screening for cross-reactivity is essential an additional adsorption of the reagent may required to ensure intra-assay specificity. In double radial immunodiffusion a reaction with Rat has been observed.

Characteristics: The reactivity of the antiserum is directed to the subclass IgG2a and IgG2b as tested against mouse sera and a panel of purified homogenous immunoglobulins. It does not react with other subclasses of IgG, IgG/Fab fragments, IgM and IgA or any non-Ig protein in mouse serum, as tested by immunoelectrophoresis and double radial immunodiffusion. As unlabelled primary or secondary reagent for indirect detection of IgG2 at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates, to prepare conjugates of the user's own choice, to prepare an insoluble immunoaffinity adsorbent or a solid phase antibody reagent by coupling to an artificial carrier and as catching antibody in non-isotopic methodology and solid phase immunochemistry. When applied in any cytochemical or histochemical staining procedure or solid phase coupling technique, the optimum concentration of the IgG preparation should be established by titration before being used.

Purification: Purified IgG fraction from antiserum

Immunogen: Pools of purified homogenous IgG2a and IgG2b isolated from mouse serum of strains belonging to the allelic types Igh-1a an Igh-1b. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune goat IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized IgG (7S) fraction is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: IgG2

Target Type: Antibody