Rabbit anti-Mouse IgG1 (Subclass specific) Antibody - Preadsorbed

Catalog No. ABIN458529

Quick Overview for Rabbit anti-Mouse IgG1 (Subclass specific) Antibody - Preadsorbed (ABIN458529)

Target: IgG1

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Dot Blot (DB), Indirect Immunofluorescence Assay (IFA)

Isotype: IgG

Product Details

Binding Specificity: Subclass specific

Reactivity: Mouse

Host: Rabbit

Purpose: Rabbit anti Mouse IgG1 (subclass specific)

Specificity: Purified IgG fraction of polyclonal Rabbit antiSerum to Mouse IgG1, subclass specific

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since immunoglobulins of different species frequently share antigenic determinants. Cross-reactivity of this product has not been tested in detail

Characteristics: The defined antibody specificity is directed to IgG1 as tested against mouse sera and a panel of purified homogenous immunoglobulins and at the level of sensitivity of direct immunofluorescence staining methods applied to lymphoid cells with the FITC conjugated form of this product. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against appropriate concentrations of the immunogen, a single characteristic precipitin line is obtained which shows a reaction of identity with the precipitin lines obtained against mouse serum and the purified monoclonal IgG1. The cytochemical grade of RAM/IgG1/7S allows its use in different types of highly sensitive immunoassays on appropriately treated cell and tissue substrates, in radioimmunoassay, for the production of immunoconjugates with a selected marker, to prepare immunoaffinity adsorbents by coupling to an artificial carrier, in non-isotopic methodology based on solid phase immunochemistry (e.g. ELISA), both as catching antibody and detection reagent, in Western blotting. This product is not pre-diluted. The optimum working dilution of each product should be established by titration before being used.

Purification: Purified IgG fraction from antiserum

Immunogen: Pools of highly purified homogenous IgG1 isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Indirect immunofluorescence,ELISA,Dot blot,Immunoblotting.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20 °C.

Buffer: Purified hyperimmune rabbit IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the immunoconjugate.

References

Kim, Cao, Finkelstein, Cárdenas, Erickson, Mehta: "A two-colour multiplexed lateral flow immunoassay system to differentially detect human malaria species on a single test line." in: Malaria journal, Vol. 18, Issue 1, pp. 313, (2019) (PubMed).

Target Details

Target: IgG1

Target Type: Antibody