Goat anti-Rat IgA, IgG, IgM (Heavy & Light Chain) Antibody

Catalog No. ABIN458645

Quick Overview for Goat anti-Rat IgA, IgG, IgM (Heavy & Light Chain) Antibody (ABIN458645)

Target: IgA, IgG, IgM

Clonality: Polyclonal

Application: Immunoelectrophoresis (IEP), Radial Immunodiffusion (RID)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rat

Host: Goat

Purpose: Goat anti Rat IgG1 IgG2a IgG2b IgG2c IgA IgM (heavy and light chains)

Specificity: Precipitating polyclonal Goat antiSerum to Rat immunoglobulins, IgG, IgA and IgM, heavy and light chains.

Cross-Reactivity (Details): Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiSerum has not been tested in detail.

Characteristics: The reactivity of the antiserum is directed to the major isotypes of the rat immunoglobulin system (classes, subclasses and light chain types) including antibodies to common determinants, to class and subclass specific determinants and to the surface determinants of the common Fab portion, as tested by immunoelectrophoresis and double radial immunodiffusion against pooled serum and purified immunoglobulins. In immunoelectrophoresis and single and double radial immunodiffusion (Mancini, Ouchterlony) to identify and measure immunoglobulins, as secondary antibody to precipitate the immunoglobulin in normal rat serum.

Purification: Delipidated, heat inactivated lyophilized stable whole serum

Immunogen: Purified normal IgG and pools of homogenous IgA, IgM and subclasses of IgG isolated from rat serum. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: Precipitation assays. In immunoelectrophoresis use 2 μL or equivalent against 120 μL antiserum. In double radial immunodiffusion use a rosette arrangement with 10 μL antiserum in a 3 mm diameter centre well and 2 μL serum samples (neat and diluted) in 2 mm diameter peripheral wells.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is reconstituted by adding 1 mL sterile distilled water.

Buffer: Delipidated, heat inactivated, lyophilized stable whole serum No preservative added, as it may interfere with the antibody activity. No foreign protein added. Total protein and IgG concentration in the antiserum are comparable to those of pooled goat seru

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antiserum is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Working dilutions are prepared by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at +4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG + IgA + IgM

Target Type: Antibody