Goat anti-Ferret IgG Antibody (Biotin)

Catalog No. ABIN5608045

Supplier Product No.: 618-106-012

Quick Overview for Goat anti-Ferret IgG Antibody (Biotin) (ABIN5608045)

Target: IgG

Clonality: Polyclonal

Conjugate: Biotin

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Reactivity: Ferret

Host: Goat

Supplier Product No.: 618-106-012

Supplier: Rockland

Purpose: Ferret IgG (gamma chain) Antibody Biotin Conjugated

Cross-Reactivity (Details): Anti-FERRET IgG (gamma chain) (GOAT) Antibody assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Ferret IgG and Ferret Serum.  No reaction was observed against Ferret IgA or Ferret IgM.

Characteristics: Anti-Ferret IgM antibody specifically detects ferret IgM.

Purification: Anti-FERRET IgG (gamma chain) (GOAT) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Ferret IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen: Optional[Immunogen]: Ferret IgG gamma heavy chain

Application Details

Application Notes: Application Note: Anti-FERRET IgG (gamma chain) (GOAT) Antibody is suitable for immunoblotting (western or dot blot), ELISA, and immunohistochemistry requiring extremely low background levels, lot-to-lot consistency, high titer and specificity. Immunohistochemistry Dilution: 1:1,000 - 1:5,000 Western Blot Dilution: 1:2,000 - 1:10,000 ELISA Dilution: 1:20,000 - 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

References

Jackson-Thompson, Kim, Jaiswal, Scott, Jones, Morris, Fite, Laurie, Hoy, Dardzinski, Mitre: "Brugia malayi infection in ferrets - A small mammal model of lymphatic filariasis." in: PLoS neglected tropical diseases, Vol. 12, Issue 3, pp. e0006334, (2018) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75 % of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.