Goat anti-Mouse IgA, IgG, IgM Antibody (HRP)

Catalog No. ABIN5608146

Supplier Product No.: 610-103-130

Quick Overview for Goat anti-Mouse IgA, IgG, IgM Antibody (HRP) (ABIN5608146)

Target: IgA, IgG, IgM

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Isotype: IgG

Product Details

Reactivity: Mouse

Host: Goat

Supplier Product No.: 610-103-130

Supplier: Rockland

Purpose: Mouse IgG IgA IgM (H&L) Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Goat Serum. This reagent is suitable for the detection of all Mouse immunoglobulin isotypes and chain combinations.

Characteristics: Anti-Mouse IgG Peroxidase Antibody generated in goat detects reactivity to Mouse IgG.

Purification: This product was prepared from polyspecific antiserum by immunoaffinity chromatography using Mouse antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Immunogen:

Immunogen: Anti-Mouse IgG IgA and IgM whole molecule was produced by repeated immunization with Mouse IgG IgA and IgM whole molecule in goat.

Immunogen Type: Native Protein

Application Details

Application Notes: Application Note: Anti-Mouse IgG IgA and IgM Peroxidase Conjugated Antibody has been tested by ELISA and is suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Immunohistochemistry Dilution: 1:250 - 1:1,500 Western Blot Dilution: 1:500 - 1:3,000 ELISA Dilution: 1:100,000 Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 1.0 mL

Concentration: 2.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

, Preservative:0.01 % (w/v) Thimerosal

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details

Target: IgA, IgG, IgM

Alternative Name: IgG IgA IgM

Target Type: Antibody

Background: Anti-Mouse IgG IgA and IgM whole molecule antibody generated in goat detects specifically Mouse IgG IgA and IgM whole molecule. This secondary antibody anti-Mouse is ideal for investigators who routinely perform ELISA, Sandwich ELISA, titration assays, western-blot, immunoprecipitation and more generally immunoassays.