Mouse anti-Human kappa Light Chain (AA 130-235) Antibody (Biotin)

Catalog No. ABIN5692177

Quick Overview for Mouse anti-Human kappa Light Chain (AA 130-235) Antibody (Biotin) (ABIN5692177)

Target: kappa Light Chain

Clonality: Monoclonal

Conjugate: Biotin

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 190F11

Isotype: IgG2a

Product Details

Binding Specificity: AA 130-235

Reactivity: Human

Host: Mouse

Purpose: Mouse anti-Ig kappa light chain Ig,IgG

Cross-Reactivity (Details): Reacts with: human. Other species not tested yet.

Characteristics: labeled with Biotin. Biotin is a small vitamin B complex molecule. Its small size minimizes interference with antibody binding sites and allows for efficient conjugation without compromising antibody functionality. Biotin conjugated antibodies can be easily detected with Avidin/Streptavidin based secondary detection systems. The streptavidin-biotin bond is one of the strongest non-covalent biological bonds known.

Purification: purified IgG

Immunogen: Recombinant protein corresponding to AA 130 to 235 from human IgG kappa LC

Application Details

Application Notes: WB:yes , IP:not tested yet ICC:not tested yet IHC:not tested yet IHC-P:1 : 1000 up to 1 : 10000,

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μLH2O to get a 1 mg/mL solution in PBS.

Preservative: Without preservative

Handling Advice: Do not store diluted antibody solutions unless you add detergent or carrier proteins such as goat serum, BSA or others. IgG sticks to glass and plastic. Any IgG solution below 0.1 mg/mL protein will quickly adsorb and denature and thus loose activity! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses.

Storage: -20 °C,-80 °C,4 °C

Storage Comment: Antibodies should be stored at +4°C when still lyophilized. Then aliquot and store at -20°C to -80°C until use. Do not freeze!

Target Details

Target: kappa Light Chain

Alternative Name: Ig kappa light chain

Background:

Synonyms: Kappa light chain, human kappa light chain, light chain, IgG, IgE, IgA, IgD, IgM, tonsil, lymphoid tissues

Antigen Information: Ig kappa light chain is a useful marker for the classification of B-cell lymphoma