Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (DyLight 594)

Catalog No. ABIN611722

Quick Overview for Donkey anti-Goat IgG (Heavy & Light Chain) Antibody (DyLight 594) (ABIN611722)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 594

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Goat

Host: Donkey

Purpose: Donkey anti-Goat IgG (H&L) - Affinity Pure, DyLight ®594 Conjugate

Specificity: Based on IEP, this antibody reacts with: • heavy (i³) chains on goat IgG • light chains on all goat immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin goat serum immunoglobulins

Purification: Affinity purified using solid phase Goat IgG

Purity: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified Goat IgG, whole molecule

Application Details

Comment:

Country of Origin: Donkey serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is stable for up to 4 weeks at 2-8 °C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20 °C.

Storage: 4 °C

Storage Comment: Store freeze-dried powder at 2-8 °C.

Expiry Date: 4 weeks

References

McLean, Fincham, Frame: "v-Src induces tyrosine phosphorylation of focal adhesion kinase independently of tyrosine 397 and formation of a complex with Src." in: The Journal of biological chemistry, Vol. 275, Issue 30, pp. 23333-9, (2000) (PubMed).

Ruest, Roy, Shi, Mernaugh, Hanks: "Phosphospecific antibodies reveal focal adhesion kinase activation loop phosphorylation in nascent and mature focal adhesions and requirement for the autophosphorylation site." in: Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research, Vol. 11, Issue 1, pp. 41-8, (2000) (PubMed).

Calalb, Zhang, Polte, Hanks: "Focal adhesion kinase tyrosine-861 is a major site of phosphorylation by Src." in: Biochemical and biophysical research communications, Vol. 228, Issue 3, pp. 662-8, (1997) (PubMed).

Target Details

Target: IgG

Target Type: Antibody