Rabbit anti-Goat IgG (Heavy & Light Chain) Antibody (DyLight 550)

Catalog No. ABIN612518

Quick Overview for Rabbit anti-Goat IgG (Heavy & Light Chain) Antibody (DyLight 550) (ABIN612518)

Target: IgG

Clonality: Polyclonal

Conjugate: DyLight 550

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Goat

Host: Rabbit

Purpose: Rabbit anti-Goat IgG (H&L) - Affinity Pure, DyLight ®550 Conjugate, min x w/human, mouse, rat lgG

Specificity: Based on IEP, this antibody reacts with: • heavy (i³) chains on goat IgG • light chains on all goat immunoglobulins

Cross-Reactivity (Details): Based on IEP, no reactivity is observed to: • non-immunoglobulin goat serum immunoglobulins • IgG from human, mouse or rat

Purification: Affinity purified using solid phase Goat IgG

Purity: Affinity purified antibody is > 95 % based on SDS-PAGE. E 1 % at 280 nm = 13.0

Immunogen: Purified goat IgG, whole molecule

Application Details

Comment:

Country of Origin: Rabbit serum was obtained from healthy animals of US origin, under the care of a registered veterinarian.

DyLight is a trademark of Thermo Fisher Scientific, Inc. and its subsidiaries.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Rehydrate with 1.1 mL of deionized water and let stand 30 minutes at room temperature to dissolve. (Product has been overfilled to ensure complete recovery.) Centrifuge to remove any particulates. Prepare fresh working dilution daily.

Concentration: 1.0 mg/mL

Buffer: 10 mM Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 1 % (w/v) BSA, Protease/IgG free, contains 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Product is stable for up to 4 weeks at 2-8 °C after rehydration. For extended storage after rehydration, add an equal volume of glycerol and store at -20 °C.

Storage: 4 °C

Storage Comment: Store freeze-dried powder at 2-8 °C.

Expiry Date: 4 weeks

References

Villena, Martin, Viñas, Cormand, Iglesias, Mampel, Giralt, Villarroya: "ETS transcription factors regulate the expression of the gene for the human mitochondrial ATP synthase beta-subunit." in: The Journal of biological chemistry, Vol. 269, Issue 51, pp. 32649-54, (1995) (PubMed).

Lee, Garboczi, Thomas, Pedersen: "Mitochondrial ATP synthase. cDNA cloning, amino acid sequence, overexpression, and properties of the rat liver alpha subunit." in: The Journal of biological chemistry, Vol. 265, Issue 8, pp. 4664-9, (1990) (PubMed).

Ohta, Tomura, Matsuda, Kagawa: "Gene structure of the human mitochondrial adenosine triphosphate synthase beta subunit." in: The Journal of biological chemistry, Vol. 263, Issue 23, pp. 11257-62, (1988) (PubMed).

Target Details

Target: IgG

Target Type: Antibody