Sheep anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed

Catalog No. ABIN616118

Quick Overview for Sheep anti-Mouse IgG (Heavy & Light Chain) Antibody (HRP) - Preadsorbed (ABIN616118)

Target: IgG

Clonality: Polyclonal

Conjugate: HRP

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Sheep

Purification: Preadsorption: Solid phase absorption

Immunogen: Mouse IgG whole molecule.

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 1.0 mL of deionized water or equivalent.

Concentration: 2.0 mg/mL

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Gentamicin Sulfate as preservative and 10 mg/mL BSA (IgG and Protease free) as stabilizer.

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50 % and then aliquot contents and freeze at -28 °C or below. This antibody is stable for one month at 2-8 °C as an undiluted liquid.

References

Carvalho, Walter, Baermann-Stapel, Weller, Panne, Schenk, Schneider: "Non-invasive monitoring of immunization progress in mice via IgG from feces." in: In vivo (Athens, Greece), Vol. 26, Issue 1, pp. 63-9, (2012) (PubMed).

Target Details

Target: IgG

Target Type: Antibody

Background: Synonyms: Mouse Immunoglobulin G