Chicken anti-Mouse IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP))

Catalog No. ABIN619063

Quick Overview for Chicken anti-Mouse IgG (Heavy & Light Chain) Antibody (Alkaline Phosphatase (AP)) (ABIN619063)

Target: IgG

Clonality: Polyclonal

Conjugate: Alkaline Phosphatase (AP)

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Isotype: IgY

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse

Host: Chicken

Purpose: Chicken anti-Mouse IgG (H&L), ALP conjugated

Purification: Immunogen affinity purified

Application Details

Application Notes: 1 : 500-1 : 2000 (WB) 1 : 20-1 : 2000 (IHC), 1 : 50-1 : 5000 (ICC),

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Immunogen affinity purified IgY.

Handling Advice: Prepare working dilution prior to use and discard after use. Do not store working dilution,Be sure to mix well but without foaming

Storage: 4 °C,-20 °C

Storage Comment: Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming.

Expiry Date: 48 months

Target Details

Target: IgG

Target Type: Antibody

Background:

Background: Chicken anti-mouse IgG (H&L), ALP (alkaline phosphatase) conjugated is a secondary antibody which binds to mouse IgGs in immunological assays. Antibodies are affinity purified using solid phase mouse IgG.

Additional information: This antibody reacts with the heavy chains on mouse IgG and with the light chains on all mouse immunoglobulins based on immunoelectrophoresis.Minimum cross-reactivity is observed to non-immunoglobulin mouse serum proteins.Antibody is supplied in 30 mM triethanolamine, pH 7.2, 5 mM magnesium chloride, 0.1 mM zinc chloride, 1 % (w/v) BSA, Protease/IgG free , 0.05 % (w/v) sodium azide as preservative.