Goat anti-Human Ig Antibody (HRP)

Catalog No. ABIN619321

Quick Overview for Goat anti-Human Ig Antibody (HRP) (ABIN619321)

Target: Ig

Clonality: Polyclonal

Conjugate: HRP

Application: Immunoassay (IA)

Isotype: IgG

Product Details

Reactivity: Human

Host: Goat

Cross-Reactivity: Mouse (Murine)

Purification: affinity purified

Immunogen: Purified human Kappa Chain BAA37169.1

Application Details

Application Notes: This conjugate is suitable for all immunoassay applications. The optimal working dilution should be determined by the investigator. Suggested starting dilution(s): 1:500-1:5 000 for Immunohistochemistry 1:200-1:5000 for ELISA/Western blot

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution please add 0.55 mL of sterile water. Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily.

Handling Advice: Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Storage: 4 °C

Storage Comment: Store lyophilized material at 2-8°C.For long time storage after reconstitution, dilute the antibody solution with glycerol to a final concentration of 50% glycerol and store as liquid at -20°C, to prevent loss of enzymatic activity. For example, if you have reconstituted 0.5 mg of antibody in 0.55 ml of sterile water add 0.55 ml of glycerol. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard. Be sure to mix well but without foaming.

Target Details

Target: Ig

Alternative Name: Immunoglobulin Ig

Background: Goat anti-human kappa (k) chain is a secondary antibody conjugated to HRP, which binds to human kappa chain in immunological assays. Antibody is affinity purified using solid phase human kappa chain.