Recombinant Alpaca anti-Mouse IgG2 Antibody (Atto 488)
WB, IHC, ICC, IF, FACS
Monoclonal
14A4
Recombinant Antibody
Atto 488
single-domain Antibody (sdAb)
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- Target See all IgG2 products
- IgG2
- Antibody Type
- Recombinant Antibody
- Fragment
- single-domain Antibody (sdAb)
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Reactivity
- Mouse
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Host
- Alpaca
- Expression System
- E.coli
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Clonality
- Monoclonal
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Conjugate
- Atto 488
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow Cytometry (FACS)
- Specificity
- Mouse IgG2, does not cross-react with rabbit, guinea pig or chicken immunoglobulins
- Purification
- Produced in: E.coli
- Immunogen
- FluoTag-X2 anti-Mouse IgG2 is a species-specific FluoTag directed against IgG2a and IgG2b subtype from Mouse. Especially useful for One-step immunofluorescence (IF) and Multiplexing applications. Our anti-mouse IgG2 sdAb binds specifically and strongly to mouse IgG2a and IgG2b independently of the IgGs VH (lambda or kappa light chain) domain.
- Clone
- 14A4
- Labeling Ratio
- Two site-specifically conjugated fluorophores per sdAb.
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- Application Notes
- 1:500 (corresponding to 5 nM final concentration if the sdAb was reconstituted as proposed)
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute with 500 µL of 50 % glycerol in deionized water. We recommend including 0.1 % sodium azide as a preservative if applicable. When reconstituted with 500 µl, the concentration of single-domain antibody is 2.5 µM
- Buffer
- The single sdAb clone was lyophilized from PBS pH 7.4.
- Storage
- -20 °C/-80 °C
- Storage Comment
- Vials containing lyophilized protein can be stored at 4 °C for 6 months. We recommend reconstituting the protein with 50 % sterile glycerol including 0.1 % sodium azide as preservative if applicable. Minimize the number of freeze-thaw cycles by aliquoting the reconstituted protein. Long term storage at -80 °C for up to 6 months. Working aliquots can be stored at -20 °C for up to 4 weeks. We do not recommend storing the reconstituted protein at 4 °C.
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- Target
- IgG2
- Abstract
- IgG2 Products
- Target Type
- Antibody
- Background
- In most of the research monoclonal antibodies are obtained from mice. These animals have a rich repertoire of immunoglobulins (Ig), displaying various isotypes (IgA, IgD, IgG, IgE, and IgM). Mouse IgGs can be categorized into five subclasses IgG1, IgG2a/c, IgG2b, IgG2c, and IgG3. In addition to these subclasses, each IgG molecule can be equipped with either a lambda or kappa light chain. The most striking difference between mouse IgG2 to other subclasses of IgGs is the significantly shorter hinge region (~12 amino acids shorter).
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