Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (Cy5) - Preadsorbed

Catalog No. ABIN7446876

Quick Overview for Goat anti-Rabbit IgG (Heavy & Light Chain) Antibody (Cy5) - Preadsorbed (ABIN7446876)

Target: IgG

Clonality: Polyclonal

Conjugate: Cy5

Application: Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (IF), Immunocytochemistry (ICC)

Isotype: IgG

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Rabbit

Host: Goat

Purpose: Goat anti-Rabbit IgG Heavy and Light Chain Cross-Adsorbed Antibody Cy5® Conjugated

No Cross-Reactivity: Chicken, Cow, Horse, Human, Mouse, Pig, Rat

Cross-Reactivity (Details): Less than 1% cross reactivity to human, rat, chicken, cow, horse, pig, and mouse IgG was detected.

Characteristics: Anti-heavy- and light-chain antibodies are designed to react with the whole intact Ig molecule. Found in all body fluids and a primary means of protection against infection, an anti-H+L IgG antibody allows for some potential cross-reactivity to other Ig molecules and IgG molecules from other closely-related species.

Purification: Preadsorption: Cross-Adsorbed

Application Details

Application Notes:

IHC: 1:50 - 1:500

ICC: 1:50 - 1:500

WB: 1:1,000 - 1:15,000. 5 % non-fat dry milk in PBST or TBST is recommended for blocking and incubation of antibodies.

IF: 1:50 - 1:500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Phosphate Buffered Saline (PBS) containing 0.2 % BSA and 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: 2 - 8°C

Expiry Date: 12 months

Target Details

Target: IgG

Target Type: Antibody