Mouse anti-Human IgG4 (pFc' Region) Antibody

Catalog No. ABIN7674790

Quick Overview for Mouse anti-Human IgG4 (pFc' Region) Antibody (ABIN7674790)

Target: IgG4

Clonality: Monoclonal

Application: Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone: NI 315 (HP 6206)

Isotype: IgG1

Product Details

Binding Specificity: pFc' Region

Reactivity: Human

Host: Mouse

Purpose: Mouse MAb anti Human IgG4 (pFc subclass specific). In an ELISA against human IgG subclasses, only IgG4 gave a strong positive reaction with the mouse anti-human IgG4 antibody. Reaction with human IgG1, IgG2 and IgG3 was minimal.

Specificity: Mouse MAb anti Human IgG4 (pFc subclass specific). In an ELISA against human IgG subclasses, only IgG4 gave a strong positive reaction with the mouse anti-human IgG4 antibody. Reaction with human IgG1, IgG2 and IgG3 was minimal.

Cross-Reactivity (Details): The antiserum does not react with any other component of the Human Ig system or any other Human plasma protein as tested. This antiSerum has not been tested for cross-reactivity with other species

Characteristics: The reactivity of the antiserum is restricted to a subclass specific determinant on the pFc portion of the IgG4 Molecule as tested in indirect binding enzyme immunoassay, immunoblotting, immunoprecipitation and indirect immunoperoxidase staining of cytoplasmic Ig. To identify the presence of IgG4 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, indirect immunoperoxidase and indirect immunofluorescence staining of cytoplasmic IgG4, and immunoblotting. The optimum working dilution is an assay-related characteristic and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:50 to 1:200, in ELISA from 1:500 upwards, in Western blotting from 1:1,000 upwards. These data should be interpreted as general recommendations only.`

Purification: Delipidated, heat inactivated ascites

Immunogen: Highly purified monoclonal IgG4 isolated from pooled human serum

Application Details

Application Notes: Immunohistochemistry, immunocytochemistry, ELISA

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Delipidated, heat inactivated lyophilized stable ascites No preservative added, as it may interfere with the antibody activity. No foreign protein added. Reconstitute the lyophilized product by adding 1.0 mL sterile distilled water to obtain an IgG concentration of 1.0 mg/mL.

Storage: 4 °C,-20 °C

Storage Comment: Lyophilized - can be stored at +4°C. After reconstitution aliquots can be stored at -20C. The lyophilized product is shipped at ambient temperature and may be stored at +4°C, prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted ascites should be stored at +4°C, not refrozen, and preferably used the same day.

Target Details

Target: IgG4

Target Type: Antibody