Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Poly-HRP)

Catalog No. ABIN7849474

Quick Overview for Goat anti-Mouse IgG (Heavy & Light Chain) Antibody (Poly-HRP) (ABIN7849474)

Target: IgG

Clonality: Polyclonal

Conjugate: Poly-HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunochromatography (IC)

Product Details

Binding Specificity: Heavy & Light Chain

Reactivity: Mouse, Rabbit

Host: Goat

Purpose: Goat Polyclonal Secondary Antibody to Mouse/Rabbit IgG (H&L) HRP polymer labeled

Specificity: By immunoelectrophoresis and ELISA this antibody reacts specifically with Mouse IgG and Rabbit IgG. No antibody was detected against non immunoglobulin serum proteins.

Purification: Goat Polyclonal Secondary Antibody to Mouse/Rabbit IgG (H&L) have been cross-adsorbed against IgG from bovine, goat, horse and human. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.

Immunogen: Mouse/Rabbit IgG

Application Details

Application Notes: ELISA: 1/5000 - 1/20000, WB: 1/5000 - 1/20000, IH: 1/100 - 1/500, IC: 1/100 - 1/500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 5 mg/mL

Buffer: Liquid in 0.01M Phosphate Buffered Saline, pH 7.2, containing 1 % BSA, 50 % glycerol, 0.05 % Proclin

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details

Target: IgG

Target Type: Antibody