Rabbit anti-Human IgM Chain C (AA 391-440) Antibody

Catalog No. ABIN7862461

Quick Overview for Rabbit anti-Human IgM Chain C (AA 391-440) Antibody (ABIN7862461)

Target: IgM Chain C

Clonality: Polyclonal

Application: ELISA, Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Isotype: IgG

Product Details

Binding Specificity: AA 391-440

Reactivity: Human, Mouse, Rat

Host: Rabbit

Purpose: Rabbit Anti-IgM Chain C Polyclonal Antibody

Specificity: IgM Chain C Polyclonal Antibody detects endogenous levels of IgM Chain C.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from IgM Chain C at AA range: 391-440

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300,WB 1:500-2000,ELISA 1:10000-20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: IgM Chain C

Background: Ig mu chain C regionIgM antibodies play an important role in primary defense mechanisms. They have been shown to be involved in early recognition of external invaders like bacteria and viruses, cellular waste and modified self, as well as in recognition and elimination of precancerous and cancerous lesions. The membrane-bound form is found in the majority of normal B-cells alongside with IgD. Membrane-bound IgM induces the phosphorylation of CD79A and CD79B by the Src family of protein tyrosine kinases. It may cause death of cells by apoptosis. It is also found in soluble form, which represents about 30 % of the total serum immunoglobulins where it is found almost exclusively as a homopentamer. After the antigen binds to the B-cell receptor, the secreted form is secreted in large amounts.

Molecular Weight: 50kD

UniProt: P01871