Mouse anti-Human IgA Antibody

Catalog No. ABIN7865652

Quick Overview for Mouse anti-Human IgA Antibody (ABIN7865652)

Target: IgA

Clonality: Monoclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: EBS-huA

Isotype: IgG1 kappa

Product Details

Reactivity: Human

Host: Mouse

Purpose: anti-IgA (human) mouse monoclonal, EBS-huA, purified

Purification: affinity chromatography

Immunogen: human salivary proteins

Application Details

Application Notes:

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details

Target: IgA

Target Type: Antibody

Background: EBS-huA recognizes the third constant domain (CH3) of the alpha chain of human IgA and reacts with both IgA1 and IgA2 isotypes and not with other types of immunoglobulins. IgA type antibodies are secreted by B-cells associated with mucosal epithelia and therefore indicate malignancy if found in lymphoid infiltrates at other locations. Detection of IgA antibodies to EBV in serum may indicate nasopharyngeal carcinoma. Rising IgA anti EBV levels are associated with progression of disease. Positive control: Human mucosal membranes, saliva.