Mouse anti-Human IgA Secretory Component Antibody

Catalog No. ABIN7865653

Quick Overview for Mouse anti-Human IgA Secretory Component Antibody (ABIN7865653)

Target: IgA Secretory Component

Clonality: Monoclonal

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: SC-05

Isotype: IgG1 kappa

Product Details

Reactivity: Human, Rat

Host: Mouse

Purpose: anti-Human IgA Secretory Component mouse monoclonal, SC-05, purified

Purification: affinity chromatography

Immunogen: affinity purified human secretory component

Application Details

Application Notes:

FACS_dilution: 0.5-1.0 μg/million cells in 0.1 ml

IHC-paraffin_dilution: 1:50-1:100 (1-2 μg/mL, microwave treatment in 10 mM citrate buffer pH 6.0 recommended)

WB_dilution: 1:50-1:100 (1-2 μg/mL)

IHC-frozen_dilution: 1:50-1:100 (1-2 μg/mL)

elisa_dilution: assay dependent

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 100 μg/mL

Buffer: PBS with 0.02 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: store at 2 - 8 °C

Target Details

Target: IgA Secretory Component

Background: SC-05 reacts with a reduction resistant epitope on 80 kDa human secretory component (both free and bound to SIgA). Secretory component is differentially expressed in epithelium, thus SC-05 can identify subpopulations of epithelial cells and epithelial differentiation. Secretory component negative cell lines are not stained with SC-05. Positive control: Stomach, lung, or breast tumor.