Donkey anti-Chicken IgY Antibody (Agarose Beads)

Catalog No. ABIN7866149

Quick Overview for Donkey anti-Chicken IgY Antibody (Agarose Beads) (ABIN7866149)

Target: IgY

Fragment: Fc fragment

Conjugate: Agarose Beads

Isotype: IgY

Product Details

Reactivity: Chicken

Host: Donkey

Purpose: Donkey anti Chicken IgY Fc, conjugated with Agarose

Purification: Egg-yolk derived IgY Affinity Purified

Immunogen: A donkey was immunized with purified chicken IgY. Anti-IgY Fc was isolated from immune sera using affinity chromatography and conjugated to N- hydroxysuccinimide (NHS)-activated agarose beads. Beads were washed extensively after the blocking of the residual NHS sites.

Application Details

Application Notes: The specific activity of the anti-IgY-agarose was determined by applying 2.7 mg IgY in 24 mL PBS (0.1 mg/mL) to 1.0 mL bed volume of agarose. Two ml aliquots were collected. The total amount of IgY that bound to the column was calculated. For this lot, 1.3 mg IgY bound per ml agarose.

Restrictions: For Research Use only

Handling

Buffer: Phosphate buffered saline, pH 7.2 with 0.075 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4-8°C. DO NOT FREEZE

Target Details

Target: IgY