Mouse anti-Human IgM (Heavy Chain) Antibody

Catalog No. ABIN7879450

Quick Overview for Mouse anti-Human IgM (Heavy Chain) Antibody (ABIN7879450)

Target: IgM

Clonality: Monoclonal

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: SPM557

Isotype: IgG1, kappa

Product Details

Binding Specificity: Heavy Chain

Reactivity: Human

Host: Mouse

Purpose: IgM Antibody

Purification: Protein G affinity chromatography

Immunogen: Recombinant heavy chain of human IgM was used as the immunogen for this IgM antibody.

Application Details

Application Notes: The optimal dilution of the IgM antibody for each application should be determined by the researcher.

1. Staining of formalin-fixed tissues requires boiling tissue sections in pH 9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 minutes.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05 % sodium azide, *For IHC use only*

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store the IgM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

Target Details

Target: IgM

Target Type: Antibody

Background: Recognizes a protein of 75 kDa, identified as mu heavy chain of human immunoglobulins. It does not cross-react with alpha (IgA), gamma (IgG), epsilon (IgE), or delta (IgD), heavy chains, T-cells, monocytes, granulocytes, or erythrocytes. This mAb is useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkins lymphomas. The most common feature of these malignancies is the restricted expression of a single heavy chain class. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is clonal and therefore malignant.

UniProt: P01871