Sheep anti-Human IgA (Free & Bound), (Secretory Component) Antibody (HRP)

Catalog No. ABIN7891014

Quick Overview for Sheep anti-Human IgA (Free & Bound), (Secretory Component) Antibody (HRP) (ABIN7891014)

Target: IgA

Clonality: Polyclonal

Conjugate: HRP

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB), Immunocytochemistry (ICC)

Isotype: IgG

Product Details

Binding Specificity: Free & Bound, Secretory Component

Reactivity: Human

Host: Sheep

Specificity: Tested in immunoelectrophoresis, double radial immunodiffusion and ELISA against a panel of appropriate secretions and purified Ig isotypes. The antiserum reacts with both bound secretory component (secretory IgA) and with the free SC present in human secretions. In immunoelectrophoresis against human milk, using a high electroendosmosis agar plate, free SC is precipitated in the alpha-2 region. The immunoconjugate does not react with other molecular forms of IgA, or with any other secretory or plasma protein. In immunocytochemical and immunohistochemical staining for the detection of free SC and secretory IgA at the cellular and subcellular level by staining of appropriately treated cell and tissue substrates. In non-isotopic assay methodology (e.g. ELISA) to identify and measure SC or secretory IgA in human body fluids.

Immunogen: Secretory component can be present in Human secretions bound to secretory IgA (sIgA) and in free form. Free Human secretory component isolated from pooled milk is used for immunization. Freund's complete adjuvant is used in the first step of the immunization procedure.

Application Details

Application Notes: This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: The antibody is reconstituted by adding 1 ml sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.

Buffer: Horseradish peroxidase-coupled purified hyperimmune sheep IgG lyophilized from a solution in phosphate buffered saline (PBS, pH 7.2). No preservative added, as it may interfere with the antibody activity.

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing. Dilute only prior to immediate use
Centrifuge product if not completely clear after standing at room temperature.
Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: 4 °C/-20 °C

Storage Comment: The lyophilized conjugate can be stored at +4°C short term; prolonged storage at or below -20°C.

Target Details

Target: IgA

Target Type: Antibody

Background: Synonyms: Human Immunoglobulin A