Rabbit anti-Dog IgG (Heavy & Light Chain) Antibody

Catalog No. ABIN965029

Product Details

Target: IgG

Fragment: F(ab')2 fragment

Binding Specificity: Heavy & Light Chain

Reactivity: Dog

Host: Rabbit

Clonality: Polyclonal

Application: ELISA, Immunohistochemistry (IHC), Western Blotting (WB)

Specificity: Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Dog IgG and Dog Serum.

Purification: F(ab')2 Anti-Dog IgG (H&L) antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Dog IgG coupled to agarose beads followed by pepsin digestion and chromatographic separation.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Dog IgG and Dog Serum.  No reaction was observed against anti-Pepsin and anti-Rabbit IgG F(c).

Immunogen:

Immunogen: Dog IgG whole molecule

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:1,000 - 1:5,000

Application Note: F(ab')2 Anti-Dog IgG (H&L) Antibody is suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. The maximum amount of reagent required to stain 1 x 10E6 cells in flow cytometry is approximately 1.0 μg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. Optimal titers for other applications should be determined by the researcher. As a general guideline dilutions of 1:100 to 1:250 should be suitable for most applications.

ELISA Dilution: 1:5,000 - 1:20,000

Western Blot Dilution: 1:2,000 - 1:10,000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4 °C prior to opening. This product is stable for several weeks at 4 °C as an undiluted liquid. For extended storage aliquot contents and freeze at -24 °C or below.

Expiry Date: 12 months

Target Details

Target: IgG

Abstract: IgG Products

Target Type: Antibody

Background:

Synonyms: Rabbit F(ab')2 Anti-Dog Antibody, Rabbit Fab2 Anti-Dog Antibody, Rabbit Fab'2 Anti-Dog Antibody

Background: F(ab')2 Anti-Dog IgG (H&L) Antibody generated in rabbit detects specifically Dog IgG. This secondary Anti-Dog IgG antibody is ideal for investigators who routinely perform titration assays, microscopy and FACS analysis. Anti-Dog IgG (H&L) Antibody is ideal for investigators in Microbiology, Molecular Biology, and Immunology.