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ZC3H6 Protein (AA 1-1189) (Strep Tag)

Recombinant ZC3H6 protein expressed in Cell-free protein synthesis (CFPS).
Catalog No. ABIN3096477
starts at
$20,480.57
Plus shipping costs $50.00, if applicable $20.00 dry ice
250 μg
Shipping to: United States
Delivery in 55 to 67 Business Days

Quick Overview for ZC3H6 Protein (AA 1-1189) (Strep Tag) (ABIN3096477)

Target

ZC3H6 (Zinc Finger CCCH-Type Containing 6 (ZC3H6))

Protein Type

Recombinant

Origin

Human

Source

Cell-free protein synthesis (CFPS)

Application

ELISA, SDS-PAGE (SDS), Western Blotting (WB)

Purity

approximately 70-80 % as determined by SDS PAGE, Western Blot and analytical SEC (HPLC).

Grade

custom-made
  • Protein Characteristics

    AA 1-1189

    Purification tag / Conjugate

    This ZC3H6 protein is labelled with Strep Tag.

    Sequence

    MTDSEHAGHD REDGELEDGE IDDAGFEEIQ EKEAKENEKQ KSEKAYRKSR KKHKKEREKK KSKRRKREKH KHNSPSSDDS SDYSLDSDVE HTESSHKKRT GFYRDYDIPF TQRGHISGSY ITSKKGQHNK KFKSKEYDEY STYSDDNFGN YSDDNFGNYG QETEEDFANQ LKQYRQAKET SNIALGSSFS KESGKKQRMK GVQQGIEQRV KSFNVGRGRG LPKKIKRKER GGRTNKGPNV FSVSDDFQEY NKPGKKWKVM TQEFINQHTV EHKGKQICKY FLEGRCIKGD QCKFDHDAEL EKRKEICKFY LQGYCTKGEN CIYMHNEFPC KFYHSGAKCY QGDNCKFSHD DLTKETKKLL DKVLNTDEEL INEDERELEE LRKRGITPLP KPPPGVGLLP TPPEHFPFSD PEDDFQTDFS DDFRKIPSLF EIVVKPTVDL AHKIGRKPPA FYTSASPPGP QFQGSSPHPQ HIYSSGSSPG PGPNMSQGHS SPVMHPGSPG HHPCAGPPGL PVPQSPPLPP GPPEIVGPQN QAGVLVQPDT SLTPPSMGGA YHSPGFPGHV MKVPRENHCS PGSSYQQSPG EMQLNTNYES LQNPAEFYDN YYAQHSIHNF QPPNNSGDGM WHGEFAQQQP PVVQDSPNHG SGSDGSSTRT GHGPLPVPGL LPAVQRALFV RLTQRYQEDE EQTSTQPHRA PSKEEDDTVN WYSSSEEEEG SSVKSILKTL QKQTETLRNQ QQPSTELSTP TDPRLAKEKS KGNQVVDPRL RTIPRQDIRK PSESAPLDLR LAWDPRKLRG NGSGHIGSSV GGAKFDLHHA NAGTNVKHKR GDDDDEDTER ELREKAFLIP LDASPGIMLQ DPRSQLRQFS HIKMDITLTK PNFAKHIVWA PEDLLPVPLP KPDPVSSINL PLPPLIADQR LNRLWNTKSD LHQNTVSIDP KLAAKAKINT TNREGYLEQF GDSHGSGAKL GDPRLQKNFD PRLHRLPNTE SHQVVMKDSH ASKGAPHLPR SNPGSSQPSG AGTSNSGSGA LPPYAPKLSS SAGLPLGTST SVLSGISLYD PRDHGSSSTS ELATASSGEN SKNQKKSGGL KSSDKTEPSP GEAILPQKPS PNVGVTLEGP ADPQADVPRS SGKVQVPAVH SLPVQALTGL IRPQYSDPRQ ARQPGQGSPT PDNDPGRETD DKSLKEVFKT FDPTASPFC
    Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us..

    Characteristics

    Key Benefits:
    • Made in Germany - from design to production - by highly experienced protein experts.
    • Protein expressed with ALiCE® and purified in one-step affinity chromatography
    • State-of-the-art algorithm used for plasmid design (Gene synthesis).

    This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

    The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.


    Expression System:
    • ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    • During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Concentration:
    • The concentration of our recombinant proteins is measured using the absorbance at 280nm.
    • The protein's absorbance will be measured against its specific reference buffer.
    • We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.

    Purification

    One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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  • Application Notes

    In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

    Comment

    ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
    During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!

    Restrictions

    For Research Use only
  • Format

    Liquid

    Buffer

    The buffer composition is at the discretion of the manufacturer.
    Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein.

    Handling Advice

    Avoid repeated freeze-thaw cycles.

    Storage

    -80 °C

    Storage Comment

    Store at -80°C.

    Expiry Date

    12 months
  • Target

    ZC3H6 (Zinc Finger CCCH-Type Containing 6 (ZC3H6))

    Alternative Name

    ZC3H6

    Background

    Zinc finger CCCH domain-containing protein 6

    Molecular Weight

    131.7 kDa

    UniProt

    P61129
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