ZNF840 Protein (AA 1-716) (Strep Tag)
Quick Overview for ZNF840 Protein (AA 1-716) (Strep Tag) (ABIN3076393)
Target
Protein Type
Origin
Source
Application
Purity
Grade
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Protein Characteristics
- AA 1-716
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Purification tag / Conjugate
- This ZNF840 protein is labelled with Strep Tag.
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Sequence
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MSLMGHGKVE MLLYAVPLLK APNCLSSSMQ LPHGGGRHQE LVRFRDVAVV FSPEEWDHLT PEQRNLYKDV MLDNCKYLAS LGNWTYKAHV MSSLKQGKEP WMMEREVTGD PCPACQPALA TFRALNESGN AFRQSFHHGE YRTHRTFVQH YECDDCGMAF GHVSQLTGHQ KIHKVGETHE YGENTRGFRH RSSFTMLQRI CTLYKHFECN QCGETFNRPS KVIQHQSMHS GLKPYKCDVC QKAFRFLSSL SIHQRFHVGN RVNLTRHQKT HTQRKPFSCN FCGKTFHRFS EKTQHLLIHT RKKYYTCNYC KKEFNPYSKF ILHQRTHTGE KPHKCDVCEK SFKSISNLNK HQKTHTGEKP FSCNECKKTF AQRTDLARHQ QIHTGKKSFI CSSCKKTFVR LSDLTQHKGT HTGERPYQCT TCEKAFKYRS NFTKHQKTHS IGRPFACNEC GKTYRLNWEL NQHKKIHTGE KPYECGECGK RFNNNSNLNK HKKIHTGEKH FVCNQCGKAF SLNSKLSRHQ RTHNKKENSS KSVSNLNKHQ KTHAGEKPFP CNECKKAFAQ RMDLARHQQI HTGRKPFICS SCKKTFVRLS DLTQHKGTHT GERPYQCTTC EKAFKYQSNF TKHQKTHSIG RPFTCNECGK TFRLNWKLNQ HKKIHTGEKP YECGECGKCF NNNSNLSKHK KIHTGEKHFV CNQCGKAFSL NSKLSRHQIT HNKKKP
Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.. -
Characteristics
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Key Benefits:
- Made in Germany - from design to production - by highly experienced protein experts.
- Protein expressed with ALiCE® and purified in one-step affinity chromatography
- State-of-the-art algorithm used for plasmid design (Gene synthesis).
This protein is a predefined custom protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.
The big advantage of ordering our predefined custom proteins in comparison to ordering custom made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.
Expression System:- ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
- During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein!
Concentration:- The concentration of our recombinant proteins is measured using the absorbance at 280nm.
- The protein's absorbance will be measured against its specific reference buffer.
- We use the Expasy's ProtParam tool to determine the absorption coefficient of each protein.
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Purification
- One-step Strep-tag purification of proteins expressed in Almost Living Cell-Free Expression System (ALiCE®).
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Application Notes
- In addition to the applications listed above we expect the protein to work for functional studies as well. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.
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Comment
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ALiCE®, our Almost Living Cell-Free Expression System is based on a lysate obtained from Nicotiana tabacum c.v.. This contains all the protein expression machinery needed to produce even the most difficult-to-express proteins, including those that require post-translational modifications.
During lysate production, the cell wall and other cellular components that are not required for protein production are removed, leaving only the protein production machinery and the mitochondria to drive the reaction. During our lysate completion steps, the additional components needed for protein production (amino acids, cofactors, etc.) are added to produce something that functions like a cell, but without the constraints of a living system - all that's needed is the DNA that codes for the desired protein! -
Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
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The buffer composition is at the discretion of the manufacturer.
Standard Storage Buffer: PBS pH 7.4, 10 % Glycerol Might differ depending on protein. -
Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -80 °C
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Storage Comment
- Store at -80°C.
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Expiry Date
- 12 months
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- ZNF840 (Zinc Finger Protein 840 (ZNF840))
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Alternative Name
- ZNF840P
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Background
- Putative zinc finger protein 840 (Zinc finger protein 840 pseudogene),FUNCTION: May be involved in transcriptional regulation. {ECO:0000250}.
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Molecular Weight
- 83.2 kDa
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UniProt
- A6NDX5
Target
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