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anti-General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1) Antibodies

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. Additionally we are shipping General Transcription Factor IIF, Polypeptide 1, 74kDa Proteins (6) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
GTF2F1 98053 Q3THK3
GTF2F1 316123 Q6AY96
GTF2F1 2962 P35269
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Top anti-General Transcription Factor IIF, Polypeptide 1, 74kDa Antibodies at antibodies-online.com

Showing 10 out of 51 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-GTF2F1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:12500Positive Control: HT1080 cell lysate WB Suggested Anti-GTF2F1  Antibody Titration: 0.2-1 µg/mL ELISA Titer: 1:12500  Positive Control: HT1080 cell lysate  GTF2F1 is strongly supported by BioGPS gene expression data to be expressed in Human HT1080 cells 100 μL Log in to see 2 to 3 Days
$289.00
Details
Cow Rabbit Un-conjugated WB WB Suggested Anti-GTF2F1 Antibody Titration: 0.2-1 ug/mlPositive Control: K562 cell lysate WB Suggested Anti-GTF2F1  Antibody Titration: 0.2-1 µg/mL  Positive Control: K562 cell lysate  There is BioGPS gene expression data showing that GTF2F1 is expressed in K562 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Mouse Un-conjugated FACS, IF, WB 100 μL Log in to see 8 to 10 Days
$493.17
Details
Human Rabbit Un-conjugated WB 50 μg Log in to see 8 to 10 Days
$551.83
Details
Human Rabbit Un-conjugated WB GTF2F1 Antibody (Center)  western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the GTF2F1 antibody detected the GTF2F1 protein (arrow). 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Mouse Un-conjugated FACS, IF, WB HEK293T cells transfected with either RC201294 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GTF2F1 antibody (ABIN2454915), and then analyzed by flow cytometry. Anti-GTF2F1 mouse monoclonal antibody (ABIN2454915) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GTF2F1 (RC201294). 100 μL Log in to see 2 to 3 Days
$423.50
Details
Human Mouse Un-conjugated FACS, IF, WB HEK293T cells transfected with either RC201294 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GTF2F1 antibody (ABIN2454912), and then analyzed by flow cytometry. Anti-GTF2F1 mouse monoclonal antibody (ABIN2454912) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GTF2F1 (RC201294). 100 μL Log in to see 2 to 3 Days
$423.50
Details
Human Mouse Un-conjugated FACS, IHC, WB HEK293T cells transfected with either RC201294 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-GTF2F1 antibody (ABIN2455071), and then analyzed by flow cytometry. HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2F1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 µg per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2F1. 100 μL Log in to see 2 to 3 Days
$482.90
Details
Human Mouse Un-conjugated FACS, IHC (p), WB 100 μL Log in to see 8 to 10 Days
$529.83
Details
Dog Rabbit Un-conjugated ELISA, WB 50 μg Log in to see 2 to 3 Days
$433.13
Details

GTF2F1 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
Mouse (Murine)
,
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Rat (Rattus)


Human , , , ,
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Top referenced anti-General Transcription Factor IIF, Polypeptide 1, 74kDa Antibodies

  1. Cow (Bovine) Polyclonal GTF2F1 Primary Antibody for WB - ABIN2780397 : Lavery, McEwan: Functional characterization of the native NH2-terminal transactivation domain of the human androgen receptor: binding kinetics for interactions with TFIIF and SRC-1a. in Biochemistry 2008 (PubMed)

  2. Cow (Bovine) Polyclonal GTF2F1 Primary Antibody for WB - ABIN2792594 : Zhang, Zobeck, Burton: Human RNA polymerase II elongation in slow motion: role of the TFIIF RAP74 alpha1 helix in nucleoside triphosphate-driven translocation. in Molecular and cellular biology 2005 (PubMed)

More Antibodies against General Transcription Factor IIF, Polypeptide 1, 74kDa Interaction Partners

Human General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1) interaction partners

  1. These results suggest that Mediator structural shifts induced by activator binding help stably orient pol II prior to transcription initiation within the human mediator-RNA polymerase II-TFIIF assembly.

  2. Gdown1 (show GRINL1A Antibodies) competes with TFIIF for binding to the RPB1 (show POLR2A Antibodies) and RPB5 (show POLR2E Antibodies) subunits of Pol II, thereby inhibiting an essential function of TFIIF in preinitiation complex assembly.

  3. Data show that TFIIF has an important role in stabilizing TFIIB (show GTF2B Antibodies) within the PIC and after transcription initiates.

  4. role of associated carboxyl-terminal domain phosphatase in dephosphorylating phosphoserines 2 and 5 of RNA polymerase II

  5. The alpha 1 helix of human RAP74 has an important role in the initiation and elongation of RNA chains

  6. The NMR solution structure of the C-terminal domain of RAP74 has been determined, and NMR methods have been used to map the binding sites of the C-terminus of CTD phosphatase/FCP1 (show CTDP1 Antibodies) on the RAP74 C-terminal fragment.

  7. cocrystal structure of the winged-helix domain of human RNA polymerase II-associating protein 74 bound to the alpha-helical C terminus of human FCP1 (show CTDP1 Antibodies)

  8. NMR structure of a complex containing this TFIIF subunit and the RNA polymerase II carboxyl-terminal domain phosphatase FCP1 (show CTDP1 Antibodies).

  9. Interaction of TFIIF subunit RAP74 with recombinant androgen receptor (show AR Antibodies) N-terminal activation domain AF1 (show EFNA5 Antibodies) leads to imposition of helical structure on the AF1 (show EFNA5 Antibodies) domain.

  10. TFIIF supports elongation and suppresses pausing by stabilizing the post-translocated elongation complex

General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1) Antigen Profile

Protein Summary

TFIIF is a general transcription initiation factor that binds to RNA polymerase II and helps to recruit it to the initiation complex in collaboration with TFIIB. It promotes transcription elongation.

Gene names and symbols associated with GTF2F1

  • general transcription factor IIF, polypeptide 1, 74kDa (GTF2F1) antibody
  • general transcription factor IIF, polypeptide 1, 74kDa (gtf2f1) antibody
  • transcription initiation factor IIF subunit alpha (AOR_1_160104) antibody
  • transcription initiation factor IIF subunit alpha (gtf2f1) antibody
  • general transcription factor IIF, polypeptide 1 (gtf2f1) antibody
  • general transcription factor IIF, polypeptide 1 (Gtf2f1) antibody
  • transcription initiation factor IIF subunit alpha (RAP74) antibody
  • 2810405L04Rik antibody
  • AO090124000085 antibody
  • ATRAP74 antibody
  • BTF4 antibody
  • C76800 antibody
  • DDBDRAFT_0205751 antibody
  • DDBDRAFT_0302526 antibody
  • DDB_0205751 antibody
  • DDB_0302526 antibody
  • fd13c08 antibody
  • RAP74 antibody
  • T1P17.200 antibody
  • T1P17_200 antibody
  • TF2F1 antibody
  • TFIIF antibody
  • wu:fd13c08 antibody
  • zgc:56155 antibody

Protein level used designations for GTF2F1

general transcription factor IIF subunit 1 , transcription initiation factor IIF alpha subunit , general transcription factor IIF, polypeptide 1, 74kDa , TFIIF-alpha , general transcription factor IIF polypeptide 1 (74kD subunit) , transcription initiation factor IIF subunit alpha , general transcription factor IIF 74 kDa subunit , transcription initiation factor RAP74 , RAP74 subunit of transcription factor IIF

GENE ID SPECIES
455639 Pan troglodytes
699221 Macaca mulatta
100124929 Xenopus (Silurana) tropicalis
100135749 Xenopus (Silurana) tropicalis
5995837 Aspergillus oryzae RIB40
8622019 Dictyostelium discoideum AX4
325832 Danio rerio
98053 Mus musculus
316123 Rattus norvegicus
2962 Homo sapiens
398325 Xenopus laevis
505702 Bos taurus
476731 Canis lupus familiaris
826874 Arabidopsis thaliana
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