GTF2F1 antibody (AA 350-400)

Catalog No. ABIN7452243

Product Details anti-GTF2F1 Antibody

Target: GTF2F1 (General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1))

Binding Specificity: AA 350-400

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GTF2F1 antibody is un-conjugated

Application: Immunoprecipitation (IP)

Purpose: Rabbit anti-RAP74 Antibody, Affinity Purified

Predicted Reactivity: Mouse,Rat,Bovine

Purification: Affinity Purified

Immunogen: between AA 350 and 400

Isotype: IgG

Application Details

Application Notes:

IP: 2 - 5 μg/mg lysate

WB: Not recommended. ABIN7452243 has not performed satisfactorily when used for WB of RAP74 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of RAP74.

Restrictions: For Research Use only

Handling

Concentration: 1000 μg/mL

Buffer: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Expiry Date: 12 months

Target Details for GTF2F1

Target: GTF2F1 (General Transcription Factor IIF, Polypeptide 1, 74kDa (GTF2F1))

Alternative Name: RAP74 (GTF2F1 Products)

Synonyms: BTF4 antibody, RAP74 antibody, TF2F1 antibody, TFIIF antibody, C76800 antibody, fd13c08 antibody, rap74 antibody, wu:fd13c08 antibody, zgc:56155 antibody, AO090124000085 antibody, DDBDRAFT_0205751 antibody, DDBDRAFT_0302526 antibody, DDB_0205751 antibody, DDB_0302526 antibody, 2810405L04Rik antibody, ATRAP74 antibody, T1P17.200 antibody, T1P17_200 antibody, general transcription factor IIF subunit 1 antibody, general transcription factor IIF, polypeptide 1 antibody, transcription initiation factor IIF subunit alpha antibody, general transcription factor IIF subunit 1 L homeolog antibody, transcription initiation factor IIF subunit alpha RAP74 antibody, GTF2F1 antibody, gtf2f1 antibody, AOR_1_160104 antibody, Gtf2f1 antibody, gtf2f1.L antibody, RAP74 antibody

Background: Background: The general transcription factor, TFIIF, is composed of a heterodimer of a 74 kDa alpha subunit and a 30 kDa beta subunit. The alpha subunit of TFIIF is also called RAP74. In collaboration with TFIIB, TFIIF helps recruit RNA polymerase II to the pre-initiation complex (PIC).

Gene ID: 2962

NCBI Accession: NP_002087

UniProt: P35269