anti-ISG15 Ubiquitin-Like Modifier (ISG15) Antibodies

The protein encoded by ISG15 is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Additionally we are shipping ISG15 Proteins (35) and ISG15 Kits (28) and many more products for this protein.

list all antibodies Gene Name GeneID UniProt
ISG15 9636 P05161
ISG15 100038882 Q64339
ISG15 298693  
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Top anti-ISG15 Antibodies at antibodies-online.com

Showing 10 out of 149 products:

Catalog No. Reactivity Host Conjugate Application Images Quantity Supplier Delivery Price Details
Cow Rabbit Un-conjugated IHC, WB <b>WB Suggested Anti-ISG15 Antibody Titration: </b>0.2-1 ug/ml<br><b>ELISA Titer: </b>1:1562500<br><b>Positive Control: </b>Human kidney <b>Sample Type</b>: 1. Human NT-2 cells (60ug)<br> 2. mouse brain extracts (80ug)<br> <b>Primary Antibody Dilution:</b> 2ug/ml<br> <b>Secondary Antibody</b>: IRDye 800CW goat anti-rabbit from Li-COR Bioscience<br> <b>Secondary Antibody Dilution:</b> 1: 20,000<br> <b>Image Submitted by</b>: Yuzhi Chen<br> University of Arkansas for Medical Science 100 μL Log in to see 2 to 3 Days
$289.00
Details
Human Rabbit Un-conjugated EIA, IHC (p), WB 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Human Rabbit Un-conjugated EIA, IHC (p) 0.4 mL Log in to see 6 to 8 Days
$390.50
Details
Human Rabbit Un-conjugated WB Western blot analysis of ISG15 Antibody (C-term N151) (ABIN387984) in HL-60 cell line lysates (35 µg/lane). ISG15 (arrow) was detected using the purified polyclonal antibody. Western blot analysis of ISG15 (arrow) using rabbit polyclonal ISG15 Antibody (C-term N151) (ABIN387984). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the ISG15 gene. 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated WB Western blot analysis of ISG15(arrow) using rabbit polyclonal ISG15 Antibody (Center R87) (ABIN387985). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with ISG15 gene (Lane 2) (Origene Technologies). 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated IHC (p), WB Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. BC = breast carcinoma. HC = hepatocarcinoma Western blot analysis of ISG15 (arrow) using rabbit polyclonal hISG15-A46 (ABIN387983). 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the ISG15 gene (Lane 2) . 400 μL Log in to see 10 to 11 Days
$324.50
Details
Human Rabbit Un-conjugated EIA, WB Immunoblot of hISG15 fusion protein. Anti-hISG15 antibody, generated by immunization with recombinant human ISG15, was tested by immunoblot against a hISG15-GFP fusion protein produced in E.coli cell lysate soluble fraction. Dilution of the antibody between 1:200 and 1:1,000 showed strong reactivity specifically with hISG15 and ISG15 coupled proteins. Free hISG15 is indicated by the arrowhead. In this blot the antibody was used at a 1:200 dilution incubated overnight at 4° C in 5% non-fat dry milk in TTBS. Detection occurred using a 1:2000 dilution of HRP-labeled Donkey anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche). Other detection systems will yield similar results. Data contributed by M. Malakhov, www.lifesensors.com, personal communication. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a, amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the modifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elonginC) -like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked head-to-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see references below. 0.5 mg Log in to see 6 to 8 Days
$488.40
Details
Human Rabbit Un-conjugated IHC (p), WB   100 μg Log in to see 4 to 6 Days
$240.00
Details
Mouse Rabbit Un-conjugated IHC (p), WB   100 μg Log in to see 4 to 6 Days
$240.00
Details
Cow Rabbit Un-conjugated IHC (p), WB 50 μg Log in to see 7 to 9 Days
$676.50
Details

ISG15 Antibodies by Reactivity, Application, Clonality and Conjugate

Attributes Applications Host Clonality Conjugate
Human , , , ,
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Mouse (Murine)


Rat (Rattus)


Top referenced anti-ISG15 Antibodies

  1. Human Polyclonal ISG15 Primary Antibody for IHC, IHC (p) - ABIN4327696 : Harris, Tabb, Sodora, Paiardini, Klatt, Douek, Silvestri, Müller-Trutwin, Vasile-Pandrea, Apetrei, Hirsch, Lifson, Brenchley, Estes: Downregulation of robust acute type I interferon responses distinguishes nonpathogenic simian immunodeficiency virus (SIV) infection of natural hosts from pathogenic SIV infection of rhesus macaques. in Journal of virology 2010 (PubMed)
    Show all 2 Pubmed References

  2. Human Polyclonal ISG15 Primary Antibody for IHC (p), WB - ABIN387983 : Padovan, Terracciano, Certa, Jacobs, Reschner, Bolli, Spagnoli, Borden, Heberer: Interferon stimulated gene 15 constitutively produced by melanoma cells induces e-cadherin expression on human dendritic cells. in Cancer research 2002 (PubMed)
    Show all 5 Pubmed References

More Antibodies against ISG15 Interaction Partners

Zebrafish ISG15 Ubiquitin-Like Modifier (ISG15) interaction partners

  1. Overall, these data demonstrate the antiviral effect of a fish ISG15 protein, revealing the conservation among vertebrates of an ISGylation mechanism likely directed against viruses.

Pig (Porcine) ISG15 Ubiquitin-Like Modifier (ISG15) interaction partners

  1. NSP2 (show RTN2 Antibodies) of porcine reproductive and respiratory syndrome virus inhibits the antiviral function of ISG15.

  2. Results report identification and cloning of the ISG15 and ISG43 (show USP18 Antibodies) genes in pig.

Human ISG15 Ubiquitin-Like Modifier (ISG15) interaction partners

  1. Results suggest that unconjugated ISG15 affects the functions of HCV NS5A through protein-protein interaction.

  2. This report demonstrates that ISG15 induced by Hepatitis E virus replication in Huh7-S10 (show L1CAM Antibodies)-3 human liver cells plays an immunomodulatory role by negatively regulating type I Interferon (show IFNA Antibodies) signaling and, thus, Hepatitis E virus sensitivity to type I Interferon (show IFNA Antibodies).

  3. epletion of either ISG15 or UBE2L6/UBCH8 (show UBE2L6 Antibodies) resulted in enhanced endogenous autophagic flux.

  4. unlike ISG15, ubiquitin and FAT10 (show UBD Antibodies) are conjugated to a similar degree to newly translated and pre-existing proteins.

  5. This paper shows that ISG15 directly regulates Human Cytomegalovirus replication and that its accumulation restricts productive virus growth.

  6. The results suggest that integrin-adhesion-induced MRTF-A (show MKL1 Antibodies)-SRF activation and ISG15 expression constitute a newly discovered signaling circuit that promotes cell migration and invasion.

  7. These data indicate that hepatic expression of PSMA6 (show PSMA6 Antibodies), which is upregulated during viral hepatitis, likely depends on TLR3 (show TLR3 Antibodies) activation and, that PSMA6 (show PSMA6 Antibodies) affects the expression of immunoregulatory ISG15, a proviral factor in the pathogenesis of hepatitis C virus infection.

  8. ISG15 dysregulation may be involved in the pathogenesis of glomerular inflammation

  9. This study shows that interferon (show IFNA Antibodies)-stimulated gene 15 (ISG15) lowers Human respiratory syncytial virus growth through protein ISGylation.

  10. Data show the induction of signal transducer and activator of transcription (STAT)1 (show STAT1 Antibodies) and IFN-stimulated gene 15 protein (ISG15) in Rickettsia conorii-infected human umbilical vein endothelial cells (HUVECs).

Mouse (Murine) ISG15 Ubiquitin-Like Modifier (ISG15) interaction partners

  1. results demonstrate that ISG15 governs the dynamic functionality of mitochondria, specifically, OXPHOS and mitophagy, broadening its physiological role as an antiviral agent.

  2. Loss of ISG15 expression and type I IFN signaling have transient effects on bacterial burden during M. tuberculosis infection in mice

  3. ISG15 acts as an immunomodulator in the cornea and plays a critical role in controlling fungal keratitis.

  4. USP18 (show USP18 Antibodies)'s ISG15 specificity is mediated by a small interaction interface.

  5. Posttranslational protein modification by ISG15 plays an important role in antiviral defense

  6. These studies provide evidence, ISG15 is elevated following TBI in mice, preceding MLCK (show MYLK Antibodies) elevation, development of BBB (show ALMS1 Antibodies) disruption, and cerebral edema.

  7. Kruppel-like factor 9 (KLF9 (show KLF9 Antibodies)) is a haploinsufficient tumor suppressor in the ApcMin/+ mouse colon by suppressing expression of ISG15, an apoptosis-inhibiting cytokine.

  8. Propose that Isg15-dependent degradation of p53 (show TP53 Antibodies) represents an alternative mechanism of controlling p53 (show TP53 Antibodies) protein levels, and, thus, it is an attractive pathway for drug discovery.

  9. Embryo mortality occurs in pregnant Isg15(-/-) mice, is exacerbated by environmental insults like maternal hypoxia, and might result from impaired early decidualization, vascular development, and formation of the labyrinth.

  10. Enhanced resistance upon influenza B infection in USP18 (show USP18 Antibodies)(C61A/C61A (show BRCC3 Antibodies)) mice was completely reversed in USP18 (show USP18 Antibodies)(C61A/C61A (show BRCC3 Antibodies)) mice, which additionally lack ISG15, providing evidence that the observed reduction in viral titers is ISG15 dependent

Cow (Bovine) ISG15 Ubiquitin-Like Modifier (ISG15) interaction partners

  1. ISG 15 gene expression is upregulated during 16-18 days of pregnancy and could be used as an early pregnancy marker in dairy cows, especially in heifers.

  2. ISG15, UBE1l (show UBA7 Antibodies) and UBCH8 (show UBE2E2 Antibodies) genes are significantly upregulated in the artificially inseminated pregnant cows.

  3. Data indicate that the expression profiles of ISG15, MX1 (show MX1 Antibodies), MX2 (show MX2 Antibodies), and OAS1 (show OAS1 Antibodies) could be a useful diagnostic biomarker of gestation.

  4. ISG15 is an antiviral and inducible protein in bovine immunodeficiency virus infected bovin lung cells.

  5. ISG15 and conjugated proteins were expressed in corpus luteum of both cyclic and pregnant cows regardless of pregnancy status and were upregulated during early pregnancy

  6. interferon-stimulated protein (show ISG20 Antibodies)(ISG15) was localized throughout the endometrium on day 18-23 of bovine pregnancy and was specifically localized to organelles and compartments of endometrial epithelial cells and stromal cells

  7. rbovISG15 is unstable over time in storage & dialysis. In vivo, conjugation of ISG15 to targeted proteins occurs inside the cell.

ISG15 Antigen Profile

Protein Summary

The protein encoded by this gene is a ubiquitin-like protein that is conjugated to intracellular target proteins upon activation by interferon-alpha and interferon-beta. Several functions have been ascribed to the encoded protein, including chemotactic activity towards neutrophils, direction of ligated target proteins to intermediate filaments, cell-to-cell signaling, and antiviral activity during viral infections. While conjugates of this protein have been found to be noncovalently attached to intermediate filaments, this protein is sometimes secreted.

Gene names and symbols associated with ISG15

  • ISG15 ubiquitin-like modifier (isg15) antibody
  • ISG15 ubiquitin-like modifier (ISG15) antibody
  • ISG15 ubiquitin-like modifier (Isg15) antibody
  • G1p2 antibody
  • hUCRP antibody
  • IFI15 antibody
  • IGI15 antibody
  • IP17 antibody
  • Irfp antibody
  • ISG15 antibody
  • si:rp71-1c23.2 antibody
  • UCRP antibody
  • zfISG15 antibody

Protein level used designations for ISG15

ISG15 ubiquitin-like modifier , interferon stimulated gene, 15 kDa , interferon, alpha-inducible protein , si:rp71-1c23.2 , zfISG15 , ubiquitin-like protein ISG15 , interferon-induced 17 kDa protein , interferon, alpha-inducible protein (clone IFI-15K) , interferon-induced 17-kDa/15-kDa protein , interferon-stimulated protein, 15 kDa , ubiquitin cross-reactive protein , interferon-stimulated protein 15 , interferon-induced 15 kDa protein , interferon-induced 15-KDa protein , interferon-stimulated protein (15 kDa) , boUCRP , interferon-stimulated gene product 17

GENE ID SPECIES
286744 Danio rerio
465390 Pan troglodytes
479575 Canis lupus familiaris
558956 Danio rerio
700141 Macaca mulatta
100145895 Sus scrofa
100174787 Felis catus
100294592 Ovis aries
9636 Homo sapiens
100038882 Mus musculus
281871 Bos taurus
298693 Rattus norvegicus
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