Vimentin Antibodies

Vimentin antibody

KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

WB Image Vimentin antibody detects Vimentin protein by western blot analysis.

IHC-P Image Vimentin antibody detects Vimentin protein at cytoplasm in rat testis by immunohistochemical analysis.

IP Image Immunoprecipitation of Vimentin protein from HeLa whole cell extracts using 5 μg of Vimentin antibody, Western blot analysis was performed using Vimentin antibody, EasyBlot anti-Rabbit IgG was used as a secondary reagent.

IHC-P Image Vimentin antibody detects Vimentin protein at cytosol on mouse thymus gland by immunohistochemical analysis.

IHC-Fr Image Immunofluorescence photomicrographs of frozen sections of mouse brain.

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Vimentin, antibody (Green) at 1:500 dilution.

IHC-P Image Immunohistochemical analysis of paraffin-embedded U373 xenograft, using Vimentin, antibody at 1:500 dilution.

Effects of MCME on migration-related proteins in CL1-0 and CL1-5 cells.

Representative immunoblotting and immunofluorescent analyses for selected differentially expressed proteins identified by proteomic analysis of H9C2 cells in response to doxorubicin treatment and pre-treatment with quercetin.

Fusion progeny exhibit epithelial-mesenchymal transition(A) Representative phase-contrast microscopic images of fusion progeny and parental cells.

FOXF1 mediates reprogramming effects on fusion progeny(A) FOXF1 mRNA levels were determined by real-time PCR in fusion progeny and parent cell lines.

Expression pattern of Notch ligands and receptors in first trimester decidual tissue.

IF (cc)

MEF2B-V5 expression alters the abundance of protein from target genes and increases HEK293A cell migration.

MEF2B mutations alter the abundance of protein from MEF2B target genes and decrease cell migration.

Serum-free suspension culture induces the enrichment of Glioma stem-like cells (GSCs).

Aryl hydrocarbon receptor (AhR) expression in different cells was correlated with E-cadherin and vimentin expression and cell motility.

Level of autophagy-related protein expression in different cells was correlated with their cell motility.

Expression of epithelial-mesenchymal transition (EMT) markers in MCF-7 o/e Snail cells.

Differential expression of epithelial-mesenchymal transition (EMT) markers in MCF-7 o/e Snail cells and MCF-F o/e Snail tissues.

ZEB2-AS1 knockdown inhibits migration, invasion and EMT in HNSCC cells.

Reintroduction of exogenous ZEB2 largely attenuated the phenotypical changes induced by ZEB2-AS1 inhibition.

ZEB2-AS1 is involved for TGF-β1-induced EMT in HNSCC cells.

Lipid loading augments hypoxia mediated secretion of pro-tumorigenic factors.

ISX expression induces TWIST1 and Snail1 expression, and promotes EMTAThe expression levels of ISX, TWIST1, Snail1, fibronectin (FN1), VEGF, and E-cadherin (CDH1) mRNA were examined in a ISX-GFP inducible Tet-ON transformants at 8 h after addition of DOX.

ISX interacts with both BRD4 and PCAF in vitro and in vivoABRD4 peptide (291-314 a.

The expression of ISX, BRD4, and PCAF is correlated with lung cancer metastasisAIHC staining with ISX (brown) or PCAF (brown) antibody in lung tumors from patients with lung cancer.

Sequencing analysis of acetylated recombinant and trypsin-digested wild-type ISX using liquid chromatography-mass spectrometryA, BPCAF acetylation of ISX was detected at lysine residue 69 by liquid chromatography-tandem mass spectrometry (LC-tandem MS-MS).

Cataracts induced epithelial-mesenchymal transition (EMT) progression with significantly elevated N-cadherin and MMP9 expression in DM (+) patients.

IF (cc)

35 images

17 references

Catalog No. ABIN2854814

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Vimentin antibody (C-Term)

VIM Reactivity: Human, Rat, Mouse WB, IHC, IF Host: Rabbit Polyclonal unconjugated

Anti- Vimentin Picoband antibody, Western blotting All lanes: Anti Vimentin at 0.

Anti- Vimentin Picoband antibody, IHC(P) IHC(P): Mouse Cardiac Muscle Tissue

Anti- Vimentin Picoband antibody, IHC(P) IHC(P): Rat Cardiac Muscle Tissue

Anti- Vimentin Picoband antibody, IHC(P) IHC(P): Human Mammary Cancer Tissue

5 images

86 references

Catalog No. ABIN3042344

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Vimentin antibody (AA 1-200)

VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

Western blot analysis of extracts of various cell lines, using Vimentin antibody (ABIN3016291, ABIN3016292, ABIN3016293 and ABIN6219693) at 1:1000 dilution.

Immunofluorescence (IF) image for anti-Vimentin (VIM) (AA 1-200) antibody (ABIN3016292)

Western blot analysis of extracts of various cell lines, using VIM antibody.

Western blot analysis of extracts of various cell lines, using Vimentin antibody (ABIN3016291, ABIN3016292, ABIN3016293 and ABIN6219693) at 1:500 dilution.

Immunohistochemistry of paraffin-embedded human lung using Vimentin antibody (ABIN3016291, ABIN3016292, ABIN3016293 and ABIN6219693) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human lung cancer using Vimentin antibody (ABIN3016291, ABIN3016292, ABIN3016293 and ABIN6219693) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse lung using Vimentin antibody (ABIN3016291, ABIN3016292, ABIN3016293 and ABIN6219693) at dilution of 1:100 (40x lens).

Confocal immunofluorescence analysis of U2OS cells using Vimentin Polyclonal Antibody (ABIN3016291, ABIN3016292, ABIN3016293 and ABIN6219693) at dilution of 1:100.

9 images

3 references

Catalog No. ABIN3016292

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Vimentin antibody

VIM Reactivity: Human, Rat, Mouse, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

Western blotting analysis of human vimentin using mouse monoclonal antibody VI-10 on lysates of MOLT-4 cell line (low expression), U87 cell line (positive) and Raji cell line (negative control) under non-reducing and reducing conditions.

Immunofluorescence staining (chicken erythrocytes)

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10

Immunofluorescence staining (rat basophils)

Immunocytochemistry staining of chicken postnatal erythrocytes.

ICC

Immunohistochemistry staining of human skin (paraffin sections) using anti-vimentin (VI-10).

6 images

1 reference

Catalog No. ABIN94492

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Vimentin antibody

VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

Western Blotting analysis of Vimentin in whole cell lysate of LEP-19 human fibroblast cell line (1,3) and 3T3 mouse fibroblast cell line (2,4).

Immunohistochemistry staining (paraffin sections) of vimentin in human liver using mouse monoclonal antibody VI-01 ((ABIN94491), diluted 1:400), detected with GAM IgM-Alexa Fluor488 (diluted 1:200, green), cell nuclei stained with PI (1 μg/mL, orange).

Immunofluorescence staining (mouse fibroblasts) Immunofluorescence staining of 3T3 mouse embryonal fibroblast cell line with anti-Vimentin (VI-01) Dyomics 547.

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody.

Immunoprecipitation of vimentin from HeLa cell lysate by antibody VI-10 and its detection by antibody VI-01.

Separation of HeLa cells stained using anti-Vimentin (VI-01) purified antibody (concentration in sample 5 μg/mL, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining of methanol permeabilisated cells).

Immunocytochemistry analysis of vimentin in methanol/acetone fixed murine 3T3 cells using mouse monoclonal antibody VI-01.

ICC

Anti-Vimentin Purified (VI-01) works in WB application under both reducing and non-reducing conditions.

9 images

4 references

Catalog No. ABIN94491

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Vimentin antibody

VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

All lanes : Anti-VIME Antibody at 1:4000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: K562 whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: C2C12 whole cell lysate Lysates/proteins at 20 μg per lane.

Immunohistochemical analysis of paraffin-embedded Human small intestine tissue using A performed on the Leica® BOND RXm.

Confocal immunofluorescent analysis of Vimentin Antibody with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Confocal immunofluorescent analysis of Vimentin Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

Confocal immunofluorescent analysis of Vimentin Antibody with cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).

All lanes : Anti-VIME Antibody at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: 293 whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: Jurkat whole cell lysate Lane 5: K562 whole cell lysate Lane 6: NIH/3T3 whole cell lysate Lane 7: C2C12 whole cell lysate Lysates/proteins at 20 μg per lane.

7 images

3 references

Catalog No. ABIN1881993

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Vimentin antibody

VIM Reactivity: Human WB, IHC, IF Host: Mouse Monoclonal C12 unconjugated

Detection of VIM in Porcine Heart lysate using Monoclonal Antibody to Vimentin (VIM)

Detection of Recombinant VIM, Human using Monoclonal Antibody to Vimentin (VIM)

Detection of VIM in Human HepG2 cell using Monoclonal Antibody to Vimentin (VIM)

Detection of VIM in Human Prostate Tissue using Monoclonal Antibody to Vimentin (VIM)

Detection of VIM in Human 293T cell lysate using Monoclonal Antibody to Vimentin (VIM)

13 images

Catalog No. ABIN7426163

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Vimentin antibody (C-Term)

VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

Vimentin Antibody (C-term) (ABIN389212 and ABIN2839367) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

Fluorescent confocal image of SY5Y cells stained with Vimentin (C-term) antibody.

Western blot analysis of Vimentin antibody (C-term) (ABIN389212 and ABIN2839367) in NCI- cell line lysates (35 μg/lane).

Vimentin Antibody (C-term) (ABIN389212 and ABIN2839367) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).

5 images

2 references

Catalog No. ABIN389212

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Vimentin antibody (AA 41-140)

VIM Reactivity: Human WB, ELISA, IF, IP Host: Mouse Monoclonal 3E9 unconjugated

Western Blot detection against Immunogen (36.

Immunoprecipitation of VIM transfected lysate using anti-VIM monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with VIM MaxPab rabbit polyclonal antibody.

Immunofluorescence of monoclonal antibody to VIM on U-2 OS cell.

A-549 cells were stained with VIM-FITC labeled monoclonal antibody (Green).

VIM monoclonal antibody (M01), clone 3E9 Western Blot analysis of VIM expression in HeLa .

Immunofluorescence of monoclonal antibody to VIM on HeLa cell.

Western Blot analysis of VIM expression in transfected 293T cell line by VIM monoclonal antibody (M01), clone 3E9.

7 images

Catalog No. ABIN563373

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Vimentin antibody (AA 2-466)

VIM Reactivity: Human, Rat, Mouse WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated

Western blot analysis using VIM mAb against HEK293 (1) and VIM (AA: 2-466)-hIgGFc transfected HEK293 (2) cell lysate.

Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using VIM mouse mAb with DAB staining.

Black line: Control Antigen (100 ng),Purple line: Antigen (10 ng), Blue line: Antigen (50 ng), Red line:Antigen (100 ng)

ELISA

Western blot analysis using VIM mAb against human VIM (AA: 2-466) recombinant protein.

Western blot analysis using VIM mouse mAb against Jurkat (1), K562 (2), SK-N-SH (3), SH-SY5Y (4), Hela (5), NIH/3T3 (6), C6 (7), and RAW264.

Flow cytometric analysis of Hela cells using VIM mouse mAb (green) and negative control (red).

Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using VIM mouse mAb with DAB staining.

7 images

Catalog No. ABIN7193582

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Vimentin antibody

VIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS, StM Host: Mouse Monoclonal VM452 unconjugated

Western Blot Analysis Raji cell lysate Vimentin Mouse Monoclonal Antibody (VM452).

Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin (VIM) Mouse Monoclonal Antibody (VM452) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array.

6 images

Catalog No. ABIN6940879

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Recombinant Vimentin antibody

VIM Reactivity: Human WB, IHC, StM Host: Rabbit Monoclonal VIM-1937R unconjugated Recombinant Antibody

Western Blot Analysis of human U-87 cell lysate using Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Vimentin Rabbit Recombinant Monoclonal Antibody (VIM/1937R).

6 images

Catalog No. ABIN6940885

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Vimentin antibody (AA 210-466)

VIM Reactivity: Human WB, IHC, IHC (p) Host: Mouse Monoclonal 5D7 unconjugated

Image no. 12 for anti-Vimentin (VIM) (AA 210-466) antibody (ABIN1491200)

Image no. 1 for anti-Vimentin (VIM) (AA 210-466) antibody (ABIN1491200)

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Image no. 13 for anti-Vimentin (VIM) (AA 210-466) antibody (ABIN1491200)

13 images

Catalog No. ABIN1491200

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Vimentin antibody

VIM Reactivity: Human WB, ELISA, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-RE-1 unconjugated

Western blotting analysis of human vimentin using mouse monoclonal antibody VI-RE/1 on lysates of MOLT-4 cell line (low expression), U87 cell line (positive) and Raji cell line (negative control) under non-reducing and reducing conditions.

Immunohistochemistry staining (paraffin sections) of vimentin in human liver using mouse monoclonal antibody VI-RE/1 ((ABIN94494), diluted 1:400), detected with GAM IgG-Alexa Fluor488 (diluted 1:200, green), cell nuclei stained with PI (1 μg/mL, orange).

Intracellular Flow Cytometry analysis Intracellular flow cytometry analysis of Vimentin expression in LEP-19 human fibroblast cell line using anti-human Vimentin (VI-RE/1) PE.

Intracellular flow cytometry analysis of vimentin expression in ESS-1 cells using anti-human vimentin (VI-RE/1) purified, GAM-FITC.

4 images

1 reference

Catalog No. ABIN94494

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Vimentin antibody (AA 1-101)

VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

Immunohistochemistry of paraffin-embedded human tonsil using VIM antibody.

Immunohistochemistry of paraffin-embedded human stomach using VIM antibody.

Immunofluorescence analysis of U2OS cells using VIM antibody.

4 images

2 references

Catalog No. ABIN6150108

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Vimentin antibody (AA 63-90)

VIM Reactivity: Human, Mouse WB, IF, IHC (p) Host: Rabbit Polyclonal RB15215 unconjugated

All lanes : Anti-Vimentin Antibody (S82) at 1:1000 dilution Lane 1: 293 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: U-2OS whole cell lysate Lane 4: C2C12 whole cell lysate Lane 5: NIH/3T3 whole cell lysate Lysates/proteins at 20 μg per lane.

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin Antibody (S82) (ABIN389211 and ABIN2839366) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Immunofluorescent analysis of 4 % paraformaldehyde-fixed, 0.

Western blot analysis of Vimentin-S82 (ABIN389211 and ABIN2839366) in Hela cell line lysates (35 μg/lane).

Western blot analysis of VIM(arrow) using rabbit polyclonal Vimentin Antibody (S82) (ABIN389211 and ABIN2839366).

5 images

Catalog No. ABIN389211

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Vimentin antibody (AA 152-181)

VIM Reactivity: Human WB, FACS, IHC (p) Host: Rabbit Polyclonal RB15676 unconjugated

Western blot analysis of Vimentin Antibody (Center) (ABIN389213 and ABIN2839368) in , cell line lysates (35 μg/lane).

Vimentin Antibody (Center) (ABIN389213 and ABIN2839368) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).

Western blot analysis of Vimentin (arrow) using Vimentin Antibody (Center) (ABIN389213 and ABIN2839368).

Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with Vimentin antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Vimentin Antibody (Center) (ABIN389213 and ABIN2839368) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.

5 images

Catalog No. ABIN389213

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Vimentin antibody (C-Term)

Verified VIM Reactivity: Human, Rat, Mouse WB, IHC, ELISA, IF, FACS Host: Goat Polyclonal unconjugated

(ABIN2613493) (5 μg/mL) staining of paraffin embedded Human Kidney.

ABIN2613493 (2µg/ml) staining of HeLa (A) and Jurkat (B) lysates (35µg protein in RIPA buffer).

ABIN2613493 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.

5 images

Catalog No. ABIN2613493

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Vimentin antibody (AA 2-466)

VIM Reactivity: Human IHC, StM Host: Mouse Monoclonal VIM-3736 unconjugated

Western Blot Analysis of U87 cell lysate using Vimentin Mouse Monoclonal Antibody (VIM/3736).

Analysis of Protein Array containing more than 21,000 full-length human proteins using Vimentin Mouse Monoclonal Antibody (VIM/3736) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array.

PAr

Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Vimentin Mouse Monoclonal Antibody (VIM/3736).

Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with Vimentin Mouse Monoclonal Antibody (VIM/3736).

SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (VIM/3736).

5 images

Catalog No. ABIN6940884

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