Vimentin antibody (C-Term)
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- Target See all Vimentin (VIM) Antibodies
- Vimentin (VIM)
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Binding Specificity
- C-Term
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Reactivity
- Human, Mouse, Rat
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- This Vimentin antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Flow Cytometry (FACS)
- Purpose
- Vimentin
- Sequence
- QVINETSQHH DDLE
- Cross-Reactivity
- Cow, Dog, Human, Mouse, Pig, Rat
- Purification
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Grade
- Verified
- Isotype
- IgG
- Top Product
- Discover our top product VIM Primary Antibody
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anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated
anti-Vimentin (VIM) antibodyKO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated
anti-Vimentin (VIM) antibodyVIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated
anti-Vimentin (VIM) antibodyVIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated
anti-Vimentin (VIM) antibodyVIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated
anti-Vimentin (VIM) antibodyVIM Reactivity: Human WB, IHC, ICC, IP Host: Mouse Monoclonal C8 unconjugated
anti-Vimentin (VIM) (AA 430-457), (C-Term) antibodyVIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated
anti-Vimentin (VIM) (AA 2-466) antibodyVIM Reactivity: Human, Mouse, Rat WB, IHC, ELISA, FACS Host: Mouse Monoclonal 3A1F2 unconjugated
anti-Vimentin (VIM) antibodyVIM Reactivity: Human WB, IHC, StM Host: Rabbit Monoclonal VIM-1937R unconjugated Recombinant Antibody
anti-Vimentin (VIM) antibodyVIM Reactivity: Human, Pig, Cow, Chicken, Dog, Cat, Goat WB, IHC, FACS, StM Host: Mouse Monoclonal VM452 unconjugated
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- Application Notes
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Immunohistochemistry: Paraffin embedded Human Kidney. Recommended concentration: 5 μg/mL.
Western Blot: Approx 55 kDa band observed in lysates of cell line HeLa and Jurkat and in Mouse Ovary lysates, and approx. 55-60 kDa band in Rat Ovary lysates (calculated MW of 53.7 kDa according to Human NP_003371.2, Mouse NP_035831.2 and Rat NP_112402.1).
Peptide ELISA: antibody detection limit dilution 1:4000.
- Comment
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Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. Immunofluorescence: Strong expression of the protein seen in the cytoplasm/Intermediate filaments of U2OS cells. Recommended conc
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.5 mg/mL
- Buffer
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- Target
- Vimentin (VIM)
- Alternative Name
- VIM (VIM Products)
- Synonyms
- CTRCT30 antibody, cb28 antibody, vime antibody, vim antibody, vim1 antibody, vim2 antibody, VIM antibody, Vimentin antibody, vim4 antibody, vimentin antibody, vimentin L homeolog antibody, vimentin S homeolog antibody, VIM antibody, Vim antibody, vim antibody, vim.L antibody, vim.S antibody
- Background
- VIM, vimentin, FLJ36605
- Gene ID
- 7431, 22352, 81818
- NCBI Accession
- NP_003371
- Pathways
- Caspase Cascade in Apoptosis
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