Add to Basket
|+1 404 474 4654|
|+1 888 205 9894 (TF)|
Interleukin 9 (IL9) antibody (Biotin)
|Synonyms||P40, HP40, IL-9, Il-9, IL9, il9|
Alternatives ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
|3 references available|
|Quantity||100 µg (1.0 mg/mL)|
|Price||345.40 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant human IL-9 protein.
Immunogen Type: Recombinant Protein
|Reconstitution||Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL|
This purified antibody has been tested for use in ELISA and western blotting. By western blot a band approximately 15 kDa in size corresponding to human IL-9 protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Synonyms: Interleukin-9, IL-9, IL9, T cell growth factor 3, T cell growth factor p40, TCGF 3, Mast cell growth factor, MCGF, Megakaryoblast growth factor, p40 cytokine.
|Characteristics||IL-9 is a cytokine that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin 9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins and thus connects this cytokine to various biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies on a mouse model of asthma demonstrated that this cytokine is a determining factor in the pathogenesis of bronchial hyperresponsiveness.|
|Specificity||This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This purified antibody has been heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant human IL-9 in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.|
Gene Name: IL9
ELISA Dilution: 1:20,000-1:100,000.
Immunohistochemistry Dilution: 1:1,000-1:5,000.
Western Blot Dilution: 1:2,000-1:10,000.
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Storage Comment||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Expiry Date||Expiration date is one (1) year from date of opening.|
|Research Area||Cytokines, Immunology, Virology, Inflammation, Cancer|
|Restrictions||For Research Use only|
Osterfeld, Ahrens, Strait et al.: "Differential roles for the IL-9/IL-9 receptor alpha-chain pathway in systemic and oral antigen-induced anaphylaxis." in: The Journal of allergy and clinical immunology, Vol. 125, Issue 2, pp. 469-476.e2, 2010 (PubMed).
Ciprandi: "Serum interleukin 9 in allergic rhinitis." in: Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, Vol. 104, Issue 2, pp. 180-1, 2010 (PubMed).
Zhu, Liu, Zhao et al.: "Association analysis of polymorphisms in IL-3, IL-4, IL-5, IL-9, and IL-13 with Graves' disease." in: Journal of endocrinological investigation, Vol. 33, Issue 10, pp. 751-5, 2010 (PubMed).
|Hosts||Rabbit (76), Mouse (2), Sheep (1)|
|Reactivities||Human (48), Mouse (Murine) (30), Rat (Rattus) (21), Cat (Feline) (1), Chicken (1), Cow (Bovine) (1), Dog (Canine) (1)|
|Applications||Western Blotting (WB) (47), ELISA (42), Immunofluorescence (IF) (31), Immunohistochemistry (Formalin-fixed Sections) (IHC (f)) (6), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (6), Neutralization (Neut) (6), Immunohistochemistry (IHC) (5), Functional Studies (Func) (4), Flow Cytometry (FACS) (3), Blocking Antibody (Inhibition) (2), Blocking Antibody (Inhibition) (2), Immunoelectron Microscopy (IEM) (2), Immunoprecipitation (IP) (2)|
|Conjugates||Biotin (11), HRP (4), PE (4), Alexa Fluor 350 (2), Alexa Fluor 488 (2), Alexa Fluor 555 (2), Alexa Fluor 647 (2), Cy3 (2), Cy5 (2), Cy5.5 (2), Cy7 (2), FITC (2), Gold (2), PE,Cy3 (1), PE,Cy5 (1), PE,Cy5.5 (1), PE,Cy7 (1), PE-Cy3 (1), PE-Cy5 (1), PE-Cy5.5 (1), PE-Cy7 (1)|
|Epitopes||C-Term (1), Internal Region (1)|