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Interleukin 27 (IL27) antibody (Biotin)
|Synonyms||p28, IL30, IL-27, IL-27A, IL27p28, MGC71873, RGD1561420, IL27|
Alternatives ELISA, Flow Cytometry (FACS), Fluorescence Microscopy (FM)
|1 reference available|
|Quantity||100 µg (1.0 mg/mL)|
|Price||345.40 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse IL27/p28 protein.
Immunogen Type: Recombinant Protein
|Reconstitution||Reconstitution Buffer: Restore with deionized water (or equivalent), Reconstitution Volume: 100 µL|
IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23.6 kDa in size corresponding to the mature mouse IL-27/p28 protein by western blotting in appropriate cell lysate or extract.
Synonyms: Subunit A of IL-27, IL-27A, p28 of IL-27.
|Characteristics||The cytokine Interleukin 27 (IL-27) is produced in response to inflammation. It is made by activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells. IL-27 consists of a heterodimeric combination of Epstein-Barr virus-induced molecule 3 (EBI3, or IL-27B) non-covalently linked with IL-27 p28 (or IL-27A). It is a regulator of T helper cell development and suppressor of T-cell proliferation. IL-27 has both pro- and anti-inflammatory properties. It can stimulate cytotoxic T cell activity and induce isotype switching in B-cells. It has diverse effects on innate immune cells. It induces monocytes and mast cells to secrete pro-inflammatory cytokines. When infection is present, IL-27 induces naive CD4+ T cells to proliferate and develop Th1 cell responses. As an anti-inflammatory regulator, IL-27 can inhibit Th1 or Th2 responses and restrict the strength and duration of adaptive immune responses. The IL-27 p28 subunit, a 28 kDa glycoprotein belonging to the type I cytokine family, is homologous to IL-12 p35, IL-23 p19, and IL-6. The EBI3 (Epstein-Barr virus-induced molecule 3, or IL-27B) subunit is a 34 kDa glycoprotein containing two fibronectin type III domains, and belongs to the type I cytokine receptor family. It can exist as a homodimer and can also heterodimerize with IL-12 p35. It is homologous to the p40 subunit of IL-12 and IL-23 and to the extracellular domain of IL-6 R. EBI3 can heterodimerize also with IL-12 p35, or can exist as a homodimer. The heterodimeric IL-27 receptor contains WSX-1 (TCCR) and gp130. WSX-1 is specific for IL-27, and is expressed on resting/naive CD4+ T cells, CD8+ T cells, NK cells, dendritic cells, monocytes, mast cells, and B cells. Gp130, on the other hand, functions as a subunit of the receptor complexes for at least seven other cytokines. IL-27 also promotes effector functions of NK cells and CD8+ T cells.|
|Specificity||This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. This antibody is specific for mouse IL-27/p28 protein. A BLAST analysis was used to suggest cross-reactivity with IL-27A/p28 from mouse sources based on 100% homology with the immunizing sequence. Based on 90% or greater positive homology, there is a chance of cross-reactivity to rat. Cross-reactivity with IL-27 from other sources has not been determined.|
Gene Name: IL27
ELISA Dilution: 1:20,000-1:100,000.
Immunohistochemistry Dilution: 1:1,000-1:5,000.
Western Blot Dilution: 1:2,000-1:10,000.
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free|
|Preservative||0.01% (w/v) Sodium Azide|
|Storage Comment||Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.|
|Expiry Date||Expiration date is one (1) year from date of opening.|
|Restrictions||For Research Use only|
Pflanz, Timans, Cheung et al.: "IL-27, a heterodimeric cytokine composed of EBI3 and p28 protein, induces proliferation of naive CD4(+) T cells." in: Immunity, Vol. 16, Issue 6, pp. 779-90, 2002 (PubMed).
|Hosts||Rabbit (23), Mouse (9), Rat (2)|
|Reactivities||Human (25), Mouse (Murine) (13)|
|Applications||Western Blotting (WB) (22), ELISA (16), Flow Cytometry (FACS) (8), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (4), Fluorescence Microscopy (FM) (3), Immunocytochemistry (ICC) (3), Immunofluorescence (IF) (2), ELISA (Capture) (1), ELISA (Detection) (1), ELISPOT (1)|
|Conjugates||Biotin (2), HRP (2), FITC (1), PE (1)|
|Epitopes||N-Term (4), C-Term (2), AA 141-155 (1), AA 20-70 (1), AA 95-107 (1), Center (1), Internal Region (1)|