Interleukin 27 (IL27) antibody

Details for Product No. ABIN1043835
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Antigen
Synonyms IL-27, IL-27A, IL27A, IL27p28, IL30, p28, RGD1561420, IL-27p28, Il30, IL27
Reactivity
Mouse (Murine)
(34), (23), (3), (1), (1)
Host
Rat
(35), (13), (3), (2)
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
(4), (3), (1), (1)
Application
Western Blotting (WB)
(44), (23), (12), (8), (5), (3), (3), (3), (2), (1), (1), (1)
Pubmed 1 reference available
Catalog no. ABIN1043835
Quantity 100 µg
Price
361.90 $   Plus shipping costs $45.00 and if applicable $26.40 dry ice
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Immunogen This Protein A purified monoclonal antibody was produced in rats by repeated immunizations with mature length recombinant mouse p28 protein (produced in E.coli) followed by hybridoma development.
Immunogen Type: Recombinant Protein
Clone 3H12.F10
Isotype IgG2a kappa
Specificity This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  This antibody is specific for mouse and rat p28 protein. Cross-reactivity with IL-27 from other sources has not been determined.
Characteristics Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.
Purification purified
Sterility Sterile filtered
Alternative Name IL-27/p28
Background Mouse IL-27/p28 Subunit, also known as Interleukin-30, is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), the heterodimer formed is IL-27. Mouse p28 is a proinflammatory cytokine inducing immunomodulatory effects. Current research is underway to delineate specific biological functions.
Synonyms: Interleukin-27 subunit alpha, IL-27 subunit alpha, IL-27-A cytokine, IL27-A, p28, IL-30
Gene ID 246779
NCBI Accession NP_663611.1
UniProt Q8K3I6
Application Notes IL-27 is expressed in activated antigen presenting cells including monocytes, endothelial cells, and dendritic cells, for example mouse CD4 splenocytes. This purified antibody has been tested for use in western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 26 KDa in size corresponding to the mature mouse p28 protein, a non-glycosylated polypeptide chain consisting of amino acids, by western blotting in appropriate cell lysate or extract.
Comment

Gene Name: IL27

Restrictions For Research Use only
Format Liquid
Concentration 1.0 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Storage 4 °C/-20 °C
Storage Comment Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.  Expiration date is one (1) year from date of opening.
Expiry Date 12 months
Supplier Images
anti-Interleukin 27 (IL27) antibody anti-Interleukin 27 (IL27) antibody
anti-Interleukin 27 (IL27) antibody (2) anti-Interleukin 27 (IL27) antibody (Image 2)
Product cited in: Murugaiyan, Mittal, Weiner: "Identification of an IL-27/osteopontin axis in dendritic cells and its modulation by IFN-gamma limits IL-17-mediated autoimmune inflammation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 107, Issue 25, pp. 11495-500, 2010 (PubMed).

Hosts (35), (13), (3), (2)
Reactivities (34), (23), (3), (1), (1)
Applications (44), (23), (12), (8), (5), (3), (3), (3), (2), (1), (1), (1)
Conjugates (4), (3), (1), (1)
Epitopes (5), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
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