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CDK12 antibody (Internal Region)

CRKRS Reactivity: Human, Dog, Monkey IHC, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN1048422
  • Target See all CDK12 (CRKRS) Antibodies
    CDK12 (CRKRS) (Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS))
    Binding Specificity
    • 8
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    Internal Region
    Reactivity
    • 31
    • 11
    • 4
    • 1
    • 1
    Human, Dog, Monkey
    Host
    • 28
    • 3
    Rabbit
    Clonality
    • 28
    • 3
    Polyclonal
    Conjugate
    • 16
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This CDK12 antibody is un-conjugated
    Application
    • 18
    • 17
    • 3
    • 2
    • 2
    • 1
    Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Brand
    IHC-plus™
    Specificity
    Human CDK12 / CRKRS. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except AKAP12 (55 % ).
    Predicted Reactivity
    Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog (100%) Elephant, Bovine, Rabbit (95%) Rat, Bat (90%) Mouse, Hamster, Horse (85%).
    Purification
    Immunoaffinity purified
    Immunogen
    Synthetic 20 amino acid peptide from internal region of human CDK12 / CRKRS. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Panda, Dog (100%), Elephant, Bovine, Rabbit (95%), Rat, Bat (90%), Mouse, Hamster, Horse (85%).

    Type of Immunogen: Synthetic peptide
    Top Product
    Discover our top product CRKRS Primary Antibody
  • Application Notes
    Approved: IHC, IHC-P (6 μg/mL)
    Comment

    Target Species of Antibody: Human

    Assay Procedure

    The IHC-pro Immunohistochemistry Protocol

    Tissue Preparation
    Formalin fixation and embedding in paraffin wax

    Tissue Sectioning
    Make 4-µm sections and place on pre-cleaned and charged microscope slides.
    Heat in a tissue-drying oven for 45 minutes at 60°C
    Deparaffinization
    Wash slides in 3 changes of xylene – 5 minutes each at room temperature.

    Rehydration
    Wash slides in 3 changes of 100% alcohol – 3 minutes each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 3 minutes each at room temperature.
    Wash slides in 1 change of 80% alcohol – 3 minutes at room temperature.
    Rinse slides in gentle running distilled water – 5 minutes at room temperature.

    Antigen retrieval
    Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C - 20 minutes
    Remove from heat and let stand at room temperature in buffer - 20 minutes
    Rinse in 1X TBS with Tween (TBST) – 1 minute at room temperature.

    Immunostaining
    Do not allow tissues to dry at any time during the staining procedure.
    Apply a universal protein block – 20 minutes at room temperature.
    Drain protein block from slides, apply diluted primary antibody – 45 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply a biotinylated secondary antibody (specific to the host of the primary antibody) - 30 minutes at room temperature.
    Rinse slides 1X TBST – 1 minute at room temperature.
    Apply alkaline phosphatase streptavidin – 30 minutes at room temperature.
    Rinse slides in 1X TBST - 1 minute at room temperature.
    Apply alkaline phosphatase chromogen substrate – 30 minutes at room temperature.
    Wash slides in distilled water – 1 minute at room temperature.

    Dehydrate
    This method should only be used if the chromogen substrate is alcohol insoluble.
    Wash slides in 2 changes of 80% alcohol – 1 minute each at room temperature.
    Wash slides in 2 changes of 95% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of 100% alcohol – 1 minute each at room temperature.
    Wash slides in 3 changes of xylene – 1 minute each at room temperature.
    Apply coverslip

    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    Lot specific
    Buffer
    PBS, less than 0.1 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Aliquot and store undiluted at -20°C or below for up to 1 year. Can be stored undiluted at 4°C for up to 1 month. Avoid freeze-thaw cycles.
    Expiry Date
    12 months
  • Target
    CDK12 (CRKRS) (Cdc2-Related Kinase, Arginine/serine-Rich (CRKRS))
    Alternative Name
    CDK12 / CRKRS (CRKRS Products)
    Synonyms
    AC017581 antibody, CG7597 antibody, Dmel\\CG7597 antibody, anon-WO0140519.165 antibody, cg7597 antibody, dCDK12 antibody, gi24668141 antibody, CRK7 antibody, CRKR antibody, CRKRS antibody, hCDK12 antibody, 1810022J16Rik antibody, AI646528 antibody, Crk7 antibody, Crkrs antibody, D11Ertd752e antibody, Pksc antibody, Cyclin-dependent kinase 12 antibody, cyclin dependent kinase 12 antibody, cyclin-dependent kinase 12 antibody, Cdk12 antibody, CDK12 antibody
    Background
    Name/Gene ID: CDK12
    Subfamily: CDC2/CDK
    Family: Protein Kinase

    Synonyms: CDK12, CDC2-related protein kinase 7, CRK7, Cyclin-dependent kinase 12, HCDK12, KIAA0904, CRKR, CRKRS, Pksc
    Gene ID
    51755
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