PPAR gamma 1/2 (0) (IN) antibody

Antigen: PPAR gamma 1/2 (0)

Epitope: IN

Clonality: Polyclonal

Host: Rabbit

Reactivity: Human, Cow (Bovine), Mouse (Murine), Rat (Rattus)

Application: ELISA, Western Blotting (WB), Immunoprecipitation (IP)

Catalog no.: ABIN105800

Additional Information

Alternative name: PPAR gamma 1 & 2 (IN)

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to human PPAR gamma 1 and 2. Peptide sequence: M-G-E-D-K-I-K-F-K-H-I-T-P-L

Format: Liquid (sterile filtered)

Specificity: This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase.

Application Details

Application Notes: Recommended Dilutions: This product has been assayed by ELISA against 0.1 ug of the immunizing peptide. A 1:7,000 to 1:33,000 dilution of the antibody is recommended for this assay. Note: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown.

Concentration: 1.0 mg/ml (by UV absorbance at 280 nm)

Buffer: Stabilizer: None. Preservative: 0.01% (w/v) Sodium Azide. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2.

Storage: Store vial at -20° C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of opening.

Research Area: Transcription Factors, Metabolism, Receptors

Restrictions: For Research Use only

Publications

Publications: Bruedigam, Koedam, Chiba et al.: "Evidence for multiple peroxisome proliferator-activated receptor gamma transcripts in bone: fine-tuning by hormonal regulation and mRNA stability." in: FEBS letters, Vol. 582, Issue 11, pp. 1618-24, 2008 (PubMed). (1:1000). Further details: The dilution for rabbit polyclonal antibody against PPARG was 1:1000